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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F06%3A1247%21RIV08-MZE-00027006
rdf:type
n3:Vysledek skos:Concept
dcterms:description
A selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding project “Czech Winter Rape”. All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryo production as related to particular genotypes. However, the fertile DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid (doubled h A selected set of twenty inter-line F1 hybrids of Brassica napus L. was evaluated for their capacity to produce microspore-derived doubled haploid (DH) plants subsequently utilized in the breeding project “Czech Winter Rape”. All tested genotypes were able to produce microspore-derived embryos based on the standardized protocol previously adopted for the Czech winter rape varieties/breeding lines; nevertheless, significant differences were found in the frequency of microspore-derived embryo production as related to particular genotypes. However, the fertile DH regenerants were obtained only in 12 hybrid lines of this set. The other 8 lines failed to produce fertile DH plants for several reasons, mainly due to altered morphology of microspore embryos connected with limited germination/conversion potential and due to unsuccessful colchicine treatment resulting in haploid or mixoploid (chimeric) regenerants exhibiting partial or full sterility. In general, colchicine treatment induced diploid (doubled h Zvolená skupina 20 F1 hybridů řepky ozimé byla prověřena z hlediska jejich schopnosti produkovat dihaploidní (DH) rostliny v mikrosporových kulturách, realizovaných v rámci sdružení %22Česká řepka%22. Všechny testované genotypy měly schopnost produkce mikrosporových embryí pomocí standardního protokolu, optimalizovaného pro kultivary českých linií a variet; i tak byly zjištěny významné rozdíly v embryogenní responsibilitě mezi testovanými genotypy. Fertilní DH regeneranty byly získány pouze z 12 testovaných hybridů. U ostatních 8 linií byla tvorba fertilních DH linií blokována z nejrůznějších důvodů, zejména v důsledku nevhodné morfologie získaných embryích a její návaznosti na přímou regeneraci a v důsledku nedostatečné diploidizační účinnosti in vivo aplikovaného kolchicinu, vedoucí k výskytu mixoploidních regenerantů. Přemnožené DH regeneranty byly zahrnuty do testů agronomických charakteristik v polních podmínkách.
dcterms:title
Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project “Czech Winter Rape” Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project “Czech Winter Rape” Efektivita mikrosporových kultur pro produkci dihaploidů v rámci sdružení %22Česká řepka%22
skos:prefLabel
Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project “Czech Winter Rape” Efektivita mikrosporových kultur pro produkci dihaploidů v rámci sdružení %22Česká řepka%22 Efficiency of Microspore Culture for Doubled Haploid Production in the Breeding Project “Czech Winter Rape”
skos:notation
RIV/00027006:_____/06:1247!RIV08-MZE-00027006
n4:strany
58;71
n4:aktivita
n10:P
n4:aktivity
P(1G46061), P(QE0052)
n4:cisloPeriodika
2
n4:dodaniDat
n15:2008
n4:domaciTvurceVysledku
n13:3080331
n4:druhVysledku
n7:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n16:predkladatel
n4:idSjednocenehoVysledku
473413
n4:idVysledku
RIV/00027006:_____/06:1247
n4:jazykVysledku
n17:eng
n4:klicovaSlova
microspore culture; doubled haploids; winter rape; Brassica napus L.; hybrid breeding
n4:klicoveSlovo
n5:winter%20rape n5:microspore%20culture n5:hybrid%20breeding n5:doubled%20haploids n5:Brassica%20napus%20L.
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[5039A098E726]
n4:nazevZdroje
Czech Journal of Genetics and Plant Breeding
n4:obor
n8:GE
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
5
n4:projekt
n9:1G46061 n9:QE0052
n4:rokUplatneniVysledku
n15:2006
n4:svazekPeriodika
42
n4:tvurceVysledku
Větrovcová, M. Klíma, Miroslav Macháčková, I. Griga, M. Smýkalová, I.
s:issn
1212-1975
s:numberOfPages
14