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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F05%3A1340%21RIV08-MZE-00027006
rdf:type
skos:Concept n17:Vysledek
dcterms:description
The composition of gliadins and HMW glutenin subunits was investigated in Czech registered wheat varieties by electrophoresis. The different alleles encoded at 6 gliadin loci and 3 glutenin loci were identified from a set of 80 varieties. Electrophoretic patterns of gliadins were obtained using SGE, HMW glutenin patterns using SDS PAGE. Gliadin diversity was high as 13, 5, 6, 8, 5, 10 and 9 alleles were found at Gld-1-1A, Gld-2-1A, Gld-1B, Gld-1D, Gld-6A, Gld-6B and Gld-6D, respectively. At Glu-1A, Glu-1B and Glu-1D, encoding HMW-GS, 3, 7 and 4 alleles were observed respectively. Identification of the varieties was verified according to the composition of gliadins and HMW-GS. Genetic constitution was defined; the presence of the genetic protein markers of baking quality, frost hardiness and stem rust resistance was detected. Pedigrees of the varieties were checked so that patterns of the parent varieties and their hybrid progenies were compared. Acquired characteristics of evaluated varieties can be Pomocí elektroforézy bylo studováno složení gliadinů a HMW podjednotek gluteninů u souboru 80 odrůd pšenice registrovaných v ČR. Elektroforetická spektra gliadinů byla byla získána metodou SGE a HMW gluteninová spektra metodou SDS PAGE. Diverzita gliadinů byla mnohem vyšší než diverzita HMW gluteninů. Identifikace odrůd byla provedena podle složení gliadinů a HMW gluteninů. Byla určena genetická struktura odrůd a přítomnost bílkovinných genetických markerů pekařské kvality, zimovzdornosti a odolnosti ke rzi travní. Byly rovněž kontrolovány původy odrůd pomocí elektroforézy bílkovinných genetických markerů . získané charakteristiky studovaných odrůd mohou být prakticky využity při tvorbě nových odrůd, udržování již registrovaných odrůd či v semenářství. The composition of gliadins and HMW glutenin subunits was investigated in Czech registered wheat varieties by electrophoresis. The different alleles encoded at 6 gliadin loci and 3 glutenin loci were identified from a set of 80 varieties. Electrophoretic patterns of gliadins were obtained using SGE, HMW glutenin patterns using SDS PAGE. Gliadin diversity was high as 13, 5, 6, 8, 5, 10 and 9 alleles were found at Gld-1-1A, Gld-2-1A, Gld-1B, Gld-1D, Gld-6A, Gld-6B and Gld-6D, respectively. At Glu-1A, Glu-1B and Glu-1D, encoding HMW-GS, 3, 7 and 4 alleles were observed respectively. Identification of the varieties was verified according to the composition of gliadins and HMW-GS. Genetic constitution was defined; the presence of the genetic protein markers of baking quality, frost hardiness and stem rust resistance was detected. Pedigrees of the varieties were checked so that patterns of the parent varieties and their hybrid progenies were compared. Acquired characteristics of evaluated varieties can be
dcterms:title
Diverzita gliadinů a HMW podjednotek gluteninů českých registrovaných odrůd pšenice Diversity of Gliadins and HMW Glutenin Subunits in Czech Registered Wheat Varieties Diversity of Gliadins and HMW Glutenin Subunits in Czech Registered Wheat Varieties
skos:prefLabel
Diversity of Gliadins and HMW Glutenin Subunits in Czech Registered Wheat Varieties Diversity of Gliadins and HMW Glutenin Subunits in Czech Registered Wheat Varieties Diverzita gliadinů a HMW podjednotek gluteninů českých registrovaných odrůd pšenice
skos:notation
RIV/00027006:_____/05:1340!RIV08-MZE-00027006
n3:strany
160;163
n3:aktivita
n12:P
n3:aktivity
P(QF3050), P(QF3133)
n3:cisloPeriodika
0
n3:dodaniDat
n9:2008
n3:domaciTvurceVysledku
n14:8192952 n14:5301610
n3:druhVysledku
n13:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
518420
n3:idVysledku
RIV/00027006:_____/05:1340
n3:jazykVysledku
n8:eng
n3:klicovaSlova
wheat; gliadin; glutenin; electrophoresis; variety identification
n3:klicoveSlovo
n4:wheat n4:glutenin n4:variety%20identification n4:gliadin n4:electrophoresis
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[416FD5DD19D6]
n3:nazevZdroje
Czech Journal of Genetics and Plant Breeding
n3:obor
n15:GE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n11:QF3050 n11:QF3133
n3:rokUplatneniVysledku
n9:2005
n3:svazekPeriodika
41
n3:tvurceVysledku
Šašek, Antonín Bradová, Jana
s:issn
1212-1975
s:numberOfPages
4