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Statements

Subject Item

n2:RIV%2F00027006%3A_____%2F04%3A83%21RIV08-MZE-00027006

rdf:type

skos:Concept n13:Vysledek

dcterms:description

Testovali jsme tři typy DNA markérů - RAPD, SSR a AFLP markéry založené na fluorescenčním značení na jejich schopnost odlišit genetické zdroje řepky. Prokázali jsem že i u této plodiny AFLP je z vedených markérů nejúčinnější. Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showed lhal the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape cultivars, 60 RAPD primers were tested and only eight of them (14%) detected sufficient levels of polymorphism. Five microsatellites out of fifteen tested were polymorphic, but in a111oci, except one, on1y two different alleles were detected. This resu1t indicated the limited degree of polymorphism found in Brassica napus. Each of the six tested AFLP combinations detected polymorphisms, the best combination (M-CAA/E-ACT) had 26% polymorphic peaks from a total of90 peaks and could distinguish the analysed cultivars and 4 out of 5 core lines of cultivars. The results presented show that florescence-based AFLP is Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showed lhal the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape cultivars, 60 RAPD primers were tested and only eight of them (14%) detected sufficient levels of polymorphism. Five microsatellites out of fifteen tested were polymorphic, but in a111oci, except one, on1y two different alleles were detected. This resu1t indicated the limited degree of polymorphism found in Brassica napus. Each of the six tested AFLP combinations detected polymorphisms, the best combination (M-CAA/E-ACT) had 26% polymorphic peaks from a total of90 peaks and could distinguish the analysed cultivars and 4 out of 5 core lines of cultivars. The results presented show that florescence-based AFLP is

dcterms:title

Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification AFLP založené na fluorescenci je nejvhodnějším markerovacím systémem pro identifikaci odrůd řepky Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification

skos:prefLabel

Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification Fluorescence-based AFLPs occur as the most suitable marker system for oiIseed rape cultivar identification AFLP založené na fluorescenci je nejvhodnějším markerovacím systémem pro identifikaci odrůd řepky

skos:notation

RIV/00027006:_____/04:83!RIV08-MZE-00027006

n3:strany

161;173

n3:aktivita

n5:Z

n3:aktivity

Z(MZE0002700602)

n3:cisloPeriodika

2

n3:dodaniDat

n11:2008

n3:domaciTvurceVysledku

n17:9505024

n3:druhVysledku

n16:J

n3:duvernostUdaju

n8:S

n3:entitaPredkladatele

n14:predkladatel

n3:idSjednocenehoVysledku

564614

n3:idVysledku

RIV/00027006:_____/04:83

n3:jazykVysledku

n15:eng

n3:klicovaSlova

AFLP; rapeseed identification; diversity

n3:klicoveSlovo

n9:diversity n9:AFLP n9:rapeseed%20identification

n3:kodStatuVydavatele

GB - Spojené království Velké Británie a Severního Irska

n3:kontrolniKodProRIV

[A26AC0B935FF]

n3:nazevZdroje

Journal of Applied Genetics

n3:obor

n12:GE

n3:pocetDomacichTvurcuVysledku

1

n3:pocetTvurcuVysledku

4

n3:rokUplatneniVysledku

n11:2004

n3:svazekPeriodika

45

n3:tvurceVysledku

Ovesná, Jaroslava Sobotka, R. Čurn, V. Dolanská, L.

n3:zamer

n10:MZE0002700602

s:issn

1234-1983

s:numberOfPages

13