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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F04%3A%23%23%23%231146%21RIV06-MZE-00027006
rdf:type
skos:Concept n13:Vysledek
dcterms:description
češ češ Molecular markers have been succesfully to used to characterise genetic resources of many plant species and they were employed in marker assisted selection. New high throughput technologies have been introduced recently to speed up the analysis. Potential of cDNA arrays is evaluated using two different species: barley and hop. In RICP barley is evaluated using DNA and expression cDNA arrays. Several protocols for RNA/mRNA isolation were tested. Plasmid carrying inserts corresponding to cold indiced barley cDNA were isolated and insetrs were amplified. Protocol for amplification of insters from bacteria directly was introduced and adopted. The number of 96 amplification products were spotted on the glass slides and hybridized with Cy3, Cy5 labelled cDNA prepared from cold stressed barley seedling to optimise array technology. RNA quality was verified by RT qPCR. Ubiquitin was used as a control. It is apparent, that more comparators will be required. A set of 96 DNAs was prepared to test DNA/DNA b
dcterms:title
Zpráva o řešení projektu QC 1336 za r. 2003 Zpráva o řešení projektu QC 1336 za r. 2003 Report of the projet: QC 1336 2003
skos:prefLabel
Zpráva o řešení projektu QC 1336 za r. 2003 Zpráva o řešení projektu QC 1336 za r. 2003 Report of the projet: QC 1336 2003
skos:notation
RIV/00027006:_____/04:####1146!RIV06-MZE-00027006
n4:aktivita
n14:P
n4:aktivity
P(QC1336)
n4:dodaniDat
n5:2006
n4:domaciTvurceVysledku
n16:9505024
n4:druhVysledku
n12:V
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
596258
n4:idVysledku
RIV/00027006:_____/04:####1146
n4:jazykVysledku
n10:cze
n4:klicovaSlova
DNA čipy, ječmen, diverzita, AFLP, SSR
n4:klicoveSlovo
n6:je%C4%8Dmen n6:AFLP n6:SSR n6:diverzita n6:DNA%20%C4%8Dipy
n4:kontrolniKodProRIV
[CBB1C5E31D8A]
n4:mistoVydani
VURV Praha
n4:objednatelVyzkumneZpravy
VURV Praha
n4:obor
n7:GE
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
1
n4:projekt
n15:QC1336
n4:rokUplatneniVysledku
n5:2004
n4:tvurceVysledku
Ovesná, Jaroslava
n4:verzeVyzkumneZpravy
zprava
s:numberOfPages
78
n18:isbn
N