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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F04%3A%23%23%23%23%23788%21RIV06-MZE-00027006
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Seven biotypes of kochia (Kochia scoparia (L.) Schrad.) resistant to chlorsulforon have been found in the Czech Republic since 1996. Several characteristics of these biotypes have been investigated so far. This is the first study on the molecular basis of their herbicide resistance to acetolactate synthase-inhibiting herbicides. Eight biotypes of kochia were examined for correspondence between restricion fragments of PCR amplification products and phenotypic resistance to chlorsurforon of individual plants. Two regions of the gene encoding acetolactate synthase (ALS) were amplified, digested with restriction enzymes and sequenced. The resistance to ALS-inhibiting herbicides in all Czech kochia biotypes studied was conferred by a mutation at codon 574 of the ALS gene. Analysis of the results of DNA sequencing indicated that the mutation induced a leucine for tryptophan substitution. Seven biotypes of kochia (Kochia scoparia (L.) Schrad.) resistant to chlorsulforon have been found in the Czech Republic since 1996. Several characteristics of these biotypes have been investigated so far. This is the first study on the molecular basis of their herbicide resistance to acetolactate synthase-inhibiting herbicides. Eight biotypes of kochia were examined for correspondence between restricion fragments of PCR amplification products and phenotypic resistance to chlorsurforon of individual plants. Two regions of the gene encoding acetolactate synthase (ALS) were amplified, digested with restriction enzymes and sequenced. The resistance to ALS-inhibiting herbicides in all Czech kochia biotypes studied was conferred by a mutation at codon 574 of the ALS gene. Analysis of the results of DNA sequencing indicated that the mutation induced a leucine for tryptophan substitution. Seven biotypes of kochia (Kochia scoparia (L.) Schrad.) resistant to chlorsulforon have been found in the Czech Republic since 1996. Several characteristics of these biotypes have been investigated so far. This is the first study on the molecular basis of their herbicide resistance to acetolactate synthase-inhibiting herbicides. Eight biotypes of kochia were examined for correspondence between restricion fragments of PCR amplification products and phenotypic resistance to chlorsurforon of individual plants. Two regions of the gene encoding acetolactate synthase (ALS) were amplified, digested with restriction enzymes and sequenced. The resistance to ALS-inhibiting herbicides in all Czech kochia biotypes studied was conferred by a mutation at codon 574 of the ALS gene. Analysis of the results of DNA sequencing indicated that the mutation induced a leucine for tryptophan substitution.
dcterms:title
Molecular basis of acetolactate synthase inhibitor resistance in Czech biotypes of kochia Molecular basis of acetolactate synthase inhibitor resistance in Czech biotypes of kochia Molekulární podstata rezistence českých biotypů bytele metlatého vůči inhibitorům acetolaktát syntázy
skos:prefLabel
Molekulární podstata rezistence českých biotypů bytele metlatého vůči inhibitorům acetolaktát syntázy Molecular basis of acetolactate synthase inhibitor resistance in Czech biotypes of kochia Molecular basis of acetolactate synthase inhibitor resistance in Czech biotypes of kochia
skos:notation
RIV/00027006:_____/04:#####788!RIV06-MZE-00027006
n3:strany
515;519
n3:aktivita
n16:Z
n3:aktivity
Z(MZE-M01-01-03)
n3:cisloPeriodika
0
n3:dodaniDat
n12:2006
n3:domaciTvurceVysledku
n7:1389092 n7:9015191 n7:1565737
n3:druhVysledku
n17:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
574352
n3:idVysledku
RIV/00027006:_____/04:#####788
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Kochia scoparia (L.) Schrad., herbicide resistance, acetolactate synthase inhibitors, genetic variation
n3:klicoveSlovo
n6:genetic%20variation n6:Kochia%20scoparia%20%28L.%29%20Schrad. n6:acetolactate%20synthase%20inhibitors n6:herbicide%20resistance
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[1CE1A5E526B0]
n3:nazevZdroje
Zeitschrift für Pflanzenkrankheiten & Pflazenschutz
n3:obor
n4:GA
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n12:2004
n3:svazekPeriodika
0
n3:tvurceVysledku
Mikulka, Jan Chodová, Daniela Salava, Jaroslav
n3:zamer
n9:MZE-M01-01-03
s:issn
0340-8159
s:numberOfPages
5