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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F03%3A33%21RIV08-MZE-00027006
rdf:type
n11:Vysledek skos:Concept
dcterms:description
The polymerase chain reaction (PCR) assay was successfully used to identify Cydia pomonella granulovirus (CpGV) in larvae of Cydia pomonella L. (codling moth). PCR with the primers CpGV-2A1/CpGV-2B and CpGV-3A/CpGV-3B was found suitable for detection of CpGV. The primers Cp-I/Cp-II and Cp-III/Cp-IV were able to identify the transposable element TCp3.2 in C. pomonella larvae. The presence of CpGV in the larvae from orchards, which had been infected with CpGV was tested during 2 years post infection. (pi.). CpGV was found in as many as 15% of the surviving larvae 1 year p.i. in one location. The virus was not detected in CpGV-infected orchards 2 years p.i. or in natural C. pomonella populations. This result suggests a poor persistence of CpGV in surviving C. pomonella individuals and its slow spread in a natural host population. One the other band, the presence of a transposable element, transposon TCp3.2 may correlate with virus redistribution in this insect population. The polymerase chain reaction (PCR) assay was successfully used to identify Cydia pomonella granulovirus (CpGV) in larvae of Cydia pomonella L. (codling moth). PCR with the primers CpGV-2A1/CpGV-2B and CpGV-3A/CpGV-3B was found suitable for detection of CpGV. The primers Cp-I/Cp-II and Cp-III/Cp-IV were able to identify the transposable element TCp3.2 in C. pomonella larvae. The presence of CpGV in the larvae from orchards, which had been infected with CpGV was tested during 2 years post infection. (pi.). CpGV was found in as many as 15% of the surviving larvae 1 year p.i. in one location. The virus was not detected in CpGV-infected orchards 2 years p.i. or in natural C. pomonella populations. This result suggests a poor persistence of CpGV in surviving C. pomonella individuals and its slow spread in a natural host population. One the other band, the presence of a transposable element, transposon TCp3.2 may correlate with virus redistribution in this insect population. PCR pro detekci granuloviru Cydia pomonella (CpGV) v larvách Cydia pomonella L. (obaleč jablečný) byla vyvinuta. Dále byly vyvinuty primery pro detekci transposonu TCp3.2 v larvách C. pomonella.
dcterms:title
Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population Metody PCR pro detekci Cydia pomonella granulovirus v populaci Cydia pomonella Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population
skos:prefLabel
Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population Metody PCR pro detekci Cydia pomonella granulovirus v populaci Cydia pomonella Polymerase chain reaction assay for Cydia pomonella granulovirus detection on Cydia pomonella population
skos:notation
RIV/00027006:_____/03:33!RIV08-MZE-00027006
n3:strany
153;157
n3:aktivita
n9:Z
n3:aktivity
Z(MZE-M01-01-03)
n3:cisloPeriodika
3
n3:dodaniDat
n13:2008
n3:domaciTvurceVysledku
n10:8585326 n10:6991793 n10:8113661
n3:druhVysledku
n4:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
621522
n3:idVysledku
RIV/00027006:_____/03:33
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Cydia pomonella; codling moth; Cydia pomonella granulovirus; PCR; transposon
n3:klicoveSlovo
n6:transposon n6:Cydia%20pomonella n6:Cydia%20pomonella%20granulovirus n6:codling%20moth n6:PCR
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[34B900369EC6]
n3:nazevZdroje
Acta Virologica
n3:obor
n16:GF
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n13:2003
n3:svazekPeriodika
47
n3:tvurceVysledku
Kumar, Jiban Kocourek, František Pultar, O. Stará, Jitka
n3:zamer
n17:MZE-M01-01-03
s:issn
0001-723X
s:numberOfPages
5