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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F03%3A32%21RIV08-MZE-00027006
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Pro zjištění vhodnosti různých pletiv jabloní pro detekci Apple stem grooving virus (ASGV) byly použity metody RT-PCR a ELISA. ASGV byl pomocí RT-PCR detekován u všech testovaných pletiv (kůra, dormantní pupeny, okvětní lístky a listy) v období od ledna do června. Listy během kvetení (v květnu) se ukázaly jako nejspolehlivější pletiva pro detekci ASGV pomocí obou metod (RT-PCR a ELlSA). Detekce ASGV metodou ELISA nebyla spolehlivá u listů odebraných v létě (v červnu až srpnu) a rovněž u jiných pletiv jako je kůra, dormantní pupeny a korunní plátky. The suitability of different apple tissues for Apple stem grooving virus (ASGV) detection throughout the year was checked by RT-PCR and ELISA. Detectable amounts of ASGV were generally found in all tissues (bark, dormant buds, petals and leaves) tested by RT-PCR from January to mid-June. Leaves during flowering (in May) were the most suitable tissues for the virus detection by both methods (RT-PCR and ELISA). The leaves collected in summer (June, July and August) or other tissues such as bark, dormant buds and petals were not reliable for ASGV detection by ELISA. The suitability of different apple tissues for Apple stem grooving virus (ASGV) detection throughout the year was checked by RT-PCR and ELISA. Detectable amounts of ASGV were generally found in all tissues (bark, dormant buds, petals and leaves) tested by RT-PCR from January to mid-June. Leaves during flowering (in May) were the most suitable tissues for the virus detection by both methods (RT-PCR and ELISA). The leaves collected in summer (June, July and August) or other tissues such as bark, dormant buds and petals were not reliable for ASGV detection by ELISA.
dcterms:title
Detection of Apple stem grooving virus in different tissues of apple trees throughout the year Detection of Apple stem grooving virus in different tissues of apple trees throughout the year Detekce Apple stem grooving virus v rozdílných pletivech jabloně během vegetace
skos:prefLabel
Detection of Apple stem grooving virus in different tissues of apple trees throughout the year Detection of Apple stem grooving virus in different tissues of apple trees throughout the year Detekce Apple stem grooving virus v rozdílných pletivech jabloně během vegetace
skos:notation
RIV/00027006:_____/03:32!RIV08-MZE-00027006
n3:strany
93;96
n3:aktivita
n6:Z
n3:aktivity
Z(MZE-M01-01-03)
n3:cisloPeriodika
3
n3:dodaniDat
n14:2008
n3:domaciTvurceVysledku
n11:8585326 n11:9664378 n11:3644464
n3:druhVysledku
n13:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
603254
n3:idVysledku
RIV/00027006:_____/03:32
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Apple stem grooving virus; tissue sampling; RT-PCR; ELISA; reliablility of detection
n3:klicoveSlovo
n9:ELISA n9:RT-PCR n9:Apple%20stem%20grooving%20virus n9:reliablility%20of%20detection n9:tissue%20sampling
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[9B043BD325F9]
n3:nazevZdroje
Plant Protection Science
n3:obor
n12:GF
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n14:2003
n3:svazekPeriodika
39
n3:tvurceVysledku
Svoboda, Jiří Polák, Jaroslav Kumar, Jiban
n3:zamer
n16:MZE-M01-01-03
s:issn
1212-2580
s:numberOfPages
4