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Statements

Subject Item
n2:RIV%2F00027006%3A_____%2F03%3A31%21RIV08-MZE-00027006
rdf:type
n11:Vysledek skos:Concept
dcterms:description
A rapid and effective RNA release procedure (RNA-RP) for detection of the Apple stem pitting virus (ASPV) and the Apple stem grooving virus (ASGV) in woody plants by reverse transcription-polymerase chain reaction (RT-PCR) was developed. RNA-RP released RNA into crude homogenate. RNA-RP was compared with classical phenol/chlorophorm extraction of RNA. The RT-PCR detection of ASPV and ASGV was shown to be similar by both the RNA preparation procedures. RNA-RP in contrast to the classical phenol/chloroform procedure represents a reliable and easy-to band protocol convenient for routine virus detection. Leaf tissues, especially dormant bud leaves and leaves during blossom, were shown to be the most optimal material for detection of these viruses, irrespective of the used RNA preparation procedure. A rapid and effective RNA release procedure (RNA-RP) for detection of the Apple stem pitting virus (ASPV) and the Apple stem grooving virus (ASGV) in woody plants by reverse transcription-polymerase chain reaction (RT-PCR) was developed. RNA-RP released RNA into crude homogenate. RNA-RP was compared with classical phenol/chlorophorm extraction of RNA. The RT-PCR detection of ASPV and ASGV was shown to be similar by both the RNA preparation procedures. RNA-RP in contrast to the classical phenol/chloroform procedure represents a reliable and easy-to band protocol convenient for routine virus detection. Leaf tissues, especially dormant bud leaves and leaves during blossom, were shown to be the most optimal material for detection of these viruses, irrespective of the used RNA preparation procedure. Rychlá a účinná metoda uvolnění RNA (RNA-RP) pro detekci ASPV a ASGV u jádrovin pomocí RT-PCR byla vyvinuta. RNA-RP byla porovnána s klasickou fenol-chloroformovou extrakcí RNA. Citlivost RT-PCR u obou metod extrakce byla podobná.
dcterms:title
A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction Rychlá a efektivní metoda RNA release pro detekci viru dřevin pomocí RT-PCR
skos:prefLabel
A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction Rychlá a efektivní metoda RNA release pro detekci viru dřevin pomocí RT-PCR A rapid and effective RNA release procedure for virus detection in woody plants by reverse trancription-polymerase chain reaction
skos:notation
RIV/00027006:_____/03:31!RIV08-MZE-00027006
n3:strany
147;151
n3:aktivita
n4:Z n4:P
n3:aktivity
P(GA522/00/D025), Z(MZE-M01-01-03)
n3:cisloPeriodika
3
n3:dodaniDat
n6:2008
n3:domaciTvurceVysledku
n17:8585326
n3:druhVysledku
n16:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
597097
n3:idVysledku
RIV/00027006:_____/03:31
n3:jazykVysledku
n13:eng
n3:klicovaSlova
ASPV; ASGV; RT-PCR
n3:klicoveSlovo
n14:RT-PCR n14:ASGV n14:ASPV
n3:kodStatuVydavatele
SK - Slovenská republika
n3:kontrolniKodProRIV
[CC2513982960]
n3:nazevZdroje
Acta Virologica
n3:obor
n8:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
1
n3:projekt
n12:GA522%2F00%2FD025
n3:rokUplatneniVysledku
n6:2003
n3:svazekPeriodika
47
n3:tvurceVysledku
Kumar, Jiban
n3:zamer
n15:MZE-M01-01-03
s:issn
0001-723X
s:numberOfPages
5