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Statements

Subject Item
n2:RIV%2F00023736%3A_____%2F11%3A00009520%21RIV13-MZ0-00023736
rdf:type
n13:Vysledek skos:Concept
rdfs:seeAlso
http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkType=FullRecord&UT=000298897400026
dcterms:description
In the present study, we compared three single platform methods for CD34+ hematopoietic stem cell (HSC) enumeration by flow cytometry. For this purpose, we analyzed the performance characteristics and results obtained from different HSC sources. Interlaboratory coefficients of variation (CV) for precision/reproducibility analysis varied from 4.0% to 6.7% / 6.7% to 9.2% for the low and 3.2% to 4.1% / 4.3% to 6.7%, respectively, for the high stem cell control. Correlation between methods ranged from 0.92% to 0.99%; Wilcoxon test showed no significant differences (p >0.05); Bland-Altman analysis confirmed good agreement between assays (mean bias ranging from -0.48 to 6.91). Our results demonstrate very good intralaboratory correlation and agreement between methods, confirm the major impact of single platform strategy for accurate and reproducible HSC enumeration and suggest that high interlaboratory variability could be influenced by incorrect performance of validated methods. In the present study, we compared three single platform methods for CD34+ hematopoietic stem cell (HSC) enumeration by flow cytometry. For this purpose, we analyzed the performance characteristics and results obtained from different HSC sources. Interlaboratory coefficients of variation (CV) for precision/reproducibility analysis varied from 4.0% to 6.7% / 6.7% to 9.2% for the low and 3.2% to 4.1% / 4.3% to 6.7%, respectively, for the high stem cell control. Correlation between methods ranged from 0.92% to 0.99%; Wilcoxon test showed no significant differences (p >0.05); Bland-Altman analysis confirmed good agreement between assays (mean bias ranging from -0.48 to 6.91). Our results demonstrate very good intralaboratory correlation and agreement between methods, confirm the major impact of single platform strategy for accurate and reproducible HSC enumeration and suggest that high interlaboratory variability could be influenced by incorrect performance of validated methods.
dcterms:title
Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry
skos:prefLabel
Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry Comparison of three single platform methods for CD34+ hematopoietic stem cell enumeration by flow cytometry
skos:notation
RIV/00023736:_____/11:00009520!RIV13-MZ0-00023736
n13:predkladatel
n16:ico%3A00023736
n4:aktivita
n15:V n15:I
n4:aktivity
I, V
n4:cisloPeriodika
11-12
n4:dodaniDat
n8:2013
n4:domaciTvurceVysledku
n11:9409718 n11:1459368 n11:4396278 Marinov, Iuri n11:1609165
n4:druhVysledku
n6:J
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
191290
n4:idVysledku
RIV/00023736:_____/11:00009520
n4:jazykVysledku
n18:eng
n4:klicovaSlova
flow cytometry; CD34+ cells; single platform methods
n4:klicoveSlovo
n5:flow%20cytometry n5:CD34%2B%20cells n5:single%20platform%20methods
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[256872FED388]
n4:nazevZdroje
Clinical laboratory
n4:obor
n10:FD
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
6
n4:rokUplatneniVysledku
n8:2011
n4:svazekPeriodika
57
n4:tvurceVysledku
Luxová, Alena Pohlreich, D. Cetkovský, Petr Gašová, Zdenka Tkáčová, Vlasta Marinov, Iuri
n4:wos
000298897400026
s:issn
1433-6510
s:numberOfPages
5