This HTML5 document contains 50 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
n9http://linked.opendata.cz/ontology/domain/vavai/riv/typAkce/
dctermshttp://purl.org/dc/terms/
n14http://purl.org/net/nknouf/ns/bibtex#
n17http://linked.opendata.cz/resource/domain/vavai/projekt/
n8http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n15http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n16http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00023736%3A_____%2F02%3A00000098%21RIV%2F2003%2FMZ0%2FL33003%2FN/
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n11http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n19http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n6http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00023736%3A_____%2F02%3A00000098%21RIV%2F2003%2FMZ0%2FL33003%2FN
rdf:type
skos:Concept n15:Vysledek
dcterms:description
The human multidrug resistance 1 (hMDR1) gene encodes a membrane-located protein (P-gp), which is capable to pump xenobiotics out of cells. This property makes hMDR1 potentially useful for gene therapy, since its overexpression can protect genetically modified hematopoietic cells against the effects of cytotoxic agents. However, ectopic expression of hMDR1 mRNA from retroviral vectors is compromised by an aberrant splicing which results in a production of a non-functional protein. Here we report that 1) our construct (QQ-SA) improved the functional properties of MDR1 compared with the wild type, although the aberrant splicing was not fully eliminated. 2) The other mutations tested resulted in the elimination of the aberrant splicing, however, they had a negative impact on the P-gp function. 3) The use of human packaging cells GP2-293 greatly reduced the frequency of MDR1 aberrant splicing in retroviral vectors and seems to be a valuable tool for generating retroviruses containing MDR1 cDNA. The human multidrug resistance 1 (hMDR1) gene encodes a membrane-located protein (P-gp), which is capable to pump xenobiotics out of cells. This property makes hMDR1 potentially useful for gene therapy, since its overexpression can protect genetically modified hematopoietic cells against the effects of cytotoxic agents. However, ectopic expression of hMDR1 mRNA from retroviral vectors is compromised by an aberrant splicing which results in a production of a non-functional protein. Here we report that 1) our construct (QQ-SA) improved the functional properties of MDR1 compared with the wild type, although the aberrant splicing was not fully eliminated. 2) The other mutations tested resulted in the elimination of the aberrant splicing, however, they had a negative impact on the P-gp function. 3) The use of human packaging cells GP2-293 greatly reduced the frequency of MDR1 aberrant splicing in retroviral vectors and seems to be a valuable tool for generating retroviruses containing MDR1 cDNA.
dcterms:title
Stability and expression of human MDR1 in retroviral vectors for gene therapy. Stability and expression of human MDR1 in retroviral vectors for gene therapy.
skos:prefLabel
Stability and expression of human MDR1 in retroviral vectors for gene therapy. Stability and expression of human MDR1 in retroviral vectors for gene therapy.
skos:notation
RIV/00023736:_____/02:00000098!RIV/2003/MZ0/L33003/N
n3:strany
O/17
n3:aktivita
n10:P
n3:aktivity
P(NE6689)
n3:dodaniDat
n6:2003
n3:domaciTvurceVysledku
n8:7991401 n8:7033877 n8:3453642 n8:1608762
n3:druhVysledku
n13:D
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
664792
n3:idVysledku
RIV/00023736:_____/02:00000098
n3:jazykVysledku
n11:eng
n3:klicovaSlova
P-gp; hMDR1; aberrant splicing; retroviral vector; gene therapy
n3:klicoveSlovo
n4:hMDR1 n4:gene%20therapy n4:aberrant%20splicing n4:retroviral%20vector n4:P-gp
n3:kontrolniKodProRIV
[0D27A962CB89]
n3:mistoKonaniAkce
Praha
n3:mistoVydani
Praha
n3:nazevZdroje
XIII. český a slovenský hematologický a transfuziologický kongres s mezinárodní účastí.
n3:obor
n19:FD
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n17:NE6689
n3:rokUplatneniVysledku
n6:2002
n3:tvurceVysledku
Baum, C. Žáková, Dana Fuchs, Ota Čmejla, Radek Čmejlová, Jana Jelínek, J.
n3:typAkce
n9:EUR
n3:zahajeniAkce
2002-01-01+01:00
s:numberOfPages
1
n14:hasPublisher
Agentura Carolina