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Statements

Subject Item
n2:RIV%2F00023736%3A_____%2F00%3A00000002%21RIV%2F2001%2FMZ0%2FL33001%2FN
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Gene expression changes were observed in the HEL and HL-60 cell lines after the stimulation of protoporphyrin IX synthesis by ALA administration and photodynamic process induction. Isolated ribonucleic acids were radiolabelled by reverse transcription, and the cDNA obtained was hybridized to membrane macroarrays (Clontech 7742-1) containing 588 gene probes. Besides changes in the activity of genes supposed to be involved in the programmed cell death and DNA reparation processes, increased or diminishedtranscription activity was also observed in several other genes; the reason for this phenomenon was not clear. The activation of programmed-cell-death genes appeared after the ALA load application, indicating the toxic effect of ALA. The gene expressionchanges observed in the two cell lines differed substantially, only a few of them were common for both cell lines. Gene expression changes were observed in the HEL and HL-60 cell lines after the stimulation of protoporphyrin IX synthesis by ALA administration and photodynamic process induction. Isolated ribonucleic acids were radiolabelled by reverse transcription, and the cDNA obtained was hybridized to membrane macroarrays (Clontech 7742-1) containing 588 gene probes. Besides changes in the activity of genes supposed to be involved in the programmed cell death and DNA reparation processes, increased or diminishedtranscription activity was also observed in several other genes; the reason for this phenomenon was not clear. The activation of programmed-cell-death genes appeared after the ALA load application, indicating the toxic effect of ALA. The gene expressionchanges observed in the two cell lines differed substantially, only a few of them were common for both cell lines.
dcterms:title
Genes involved in the destruction of leukaemic cells by induced photosensitivity. Genes involved in the destruction of leukaemic cells by induced photosensitivity.
skos:prefLabel
Genes involved in the destruction of leukaemic cells by induced photosensitivity. Genes involved in the destruction of leukaemic cells by induced photosensitivity.
skos:notation
RIV/00023736:_____/00:00000002!RIV/2001/MZ0/L33001/N
n4:strany
131
n4:aktivita
n5:P
n4:aktivity
P(NM5901)
n4:cisloPeriodika
4
n4:dodaniDat
n7:2001
n4:domaciTvurceVysledku
Cajthamlová, Hana Bruchová, Hana Hrkal, Zbyněk Beličková, Monika Brdička, Radim
n4:druhVysledku
n15:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n8:predkladatel
n4:idSjednocenehoVysledku
711828
n4:idVysledku
RIV/00023736:_____/00:00000002
n4:jazykVysledku
n11:eng
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[A5FC2A4293C9]
n4:nazevZdroje
Folia Biologica
n4:obor
n12:FD
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
5
n4:projekt
n13:NM5901
n4:rokUplatneniVysledku
n7:2000
n4:svazekPeriodika
46
n4:tvurceVysledku
Beličková, Monika Bruchová, Hana Hrkal, Zbyněk Brdička, Radim Cajthamlová, Hana
s:issn
0015-5500
s:numberOfPages
5