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Statements

Subject Item
n2:RIV%2F00023728%3A_____%2F14%3A%230003054%21RIV14-MZ0-00023728
rdf:type
n14:Vysledek skos:Concept
dcterms:description
Preparation of RNA is a crucial starting point in all expression analysis studies. The quality and specific template quantity of RNA can affect the amplification efficiency and also the final expression values. Authors have compared five different RNA extraction protocols from peripheral blood/PBMCs with the aim to define the most suitable method for the miRNA expression profiling experiments. They conclude that the yield and quality of total RNA as well as the portion of miRNAs prepared by the TRIzol-chloroform extraction procedure is sufficient for downstream expression profiling analysis. Preparation of RNA is a crucial starting point in all expression analysis studies. The quality and specific template quantity of RNA can affect the amplification efficiency and also the final expression values. Authors have compared five different RNA extraction protocols from peripheral blood/PBMCs with the aim to define the most suitable method for the miRNA expression profiling experiments. They conclude that the yield and quality of total RNA as well as the portion of miRNAs prepared by the TRIzol-chloroform extraction procedure is sufficient for downstream expression profiling analysis.
dcterms:title
Validation of RNA extraction procedures focused on micro RNA expression analysis Validation of RNA extraction procedures focused on micro RNA expression analysis
skos:prefLabel
Validation of RNA extraction procedures focused on micro RNA expression analysis Validation of RNA extraction procedures focused on micro RNA expression analysis
skos:notation
RIV/00023728:_____/14:#0003054!RIV14-MZ0-00023728
n14:predkladatel
n15:ico%3A00023728
n3:aktivita
n12:Z n12:P n12:I
n3:aktivity
I, P(NT12452), Z(MZ00000023728)
n3:cisloPeriodika
1
n3:dodaniDat
n5:2014
n3:domaciTvurceVysledku
n6:5778107 n6:7348134 n6:5017130 n6:9898018 n6:8827559
n3:druhVysledku
n4:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
52913
n3:idVysledku
RIV/00023728:_____/14:#0003054
n3:jazykVysledku
n17:eng
n3:klicovaSlova
RNA preparation; miRNA; RIN
n3:klicoveSlovo
n16:miRNA n16:RIN n16:RNA%20preparation
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[86B66E821225]
n3:nazevZdroje
Folia biologica
n3:obor
n10:FE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n7:NT12452
n3:rokUplatneniVysledku
n5:2014
n3:svazekPeriodika
59
n3:tvurceVysledku
Faustová, Markéta Škoda, Marek Novota, Peter Vencovský, Jiří Remáková, Martina
n3:wos
000326215200006
n3:zamer
n18:MZ00000023728
s:issn
0015-5500
s:numberOfPages
4