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Statements

Subject Item
n2:RIV%2F00023001%3A_____%2F11%3A00002530%21RIV12-MZ0-00023001
rdf:type
n9:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0041134511014084
dcterms:description
Differentiation of pancreatic progenitors into insulin-producing cells is regulated by various transcription factors. To be expressed the genes coding these transcription factors need to be in accessible DNA. Whether a particular gene is present in a form of active euchromatin structure with accessible DNA or in an inactive heterochromatin structure with inaccessible DNA is determined by various epigenetic modifications. We studied the effect of epigenetic modifiers on differentiation of human nonendocrine cells into insulin-producing cells with the aim to evaluate the effect of epigenetic modifications in that process. Within 3 days of cultivation nonendocrine cells form isletlike cell clusters (ILCCs) containing mainly cytokeratin-19-positive cells. After cultivation with epigenetic modifiers and further differentiation, the highest number of C-peptide-positive cells (10.3% 2.9%) as well as glucagon-positive cells (7.2% 2.8%) was observed in a samplesupplemented with a combination of 5-Aza-2=-deoxycytidine modifiers, BIX01294 and MC1568. In response to glucose stimulation (5 vs 20 mmol/L) these ILCCs secreted increased amounts of C-peptide (0.45 vs 1.05 pmol C-peptide/g DNA). Control samples treated without any epigenetic modifiers showed significantly lower numbers of C-peptidepositive cells (3.5% 1.6%). These results showed that a combination of epigenetic modifiers 5-Aza-2=-deoxycytidine (BIX01294 and MC1568) significantly improved reproducible differentiation of nonendocrine pancreatic cells into insulin-producing cells. Differentiation of pancreatic progenitors into insulin-producing cells is regulated by various transcription factors. To be expressed the genes coding these transcription factors need to be in accessible DNA. Whether a particular gene is present in a form of active euchromatin structure with accessible DNA or in an inactive heterochromatin structure with inaccessible DNA is determined by various epigenetic modifications. We studied the effect of epigenetic modifiers on differentiation of human nonendocrine cells into insulin-producing cells with the aim to evaluate the effect of epigenetic modifications in that process. Within 3 days of cultivation nonendocrine cells form isletlike cell clusters (ILCCs) containing mainly cytokeratin-19-positive cells. After cultivation with epigenetic modifiers and further differentiation, the highest number of C-peptide-positive cells (10.3% 2.9%) as well as glucagon-positive cells (7.2% 2.8%) was observed in a samplesupplemented with a combination of 5-Aza-2=-deoxycytidine modifiers, BIX01294 and MC1568. In response to glucose stimulation (5 vs 20 mmol/L) these ILCCs secreted increased amounts of C-peptide (0.45 vs 1.05 pmol C-peptide/g DNA). Control samples treated without any epigenetic modifiers showed significantly lower numbers of C-peptidepositive cells (3.5% 1.6%). These results showed that a combination of epigenetic modifiers 5-Aza-2=-deoxycytidine (BIX01294 and MC1568) significantly improved reproducible differentiation of nonendocrine pancreatic cells into insulin-producing cells.
dcterms:title
The effect of epigenetic factors on differentiations of pancreatic progenitor cells into insulin-producings cells The effect of epigenetic factors on differentiations of pancreatic progenitor cells into insulin-producings cells
skos:prefLabel
The effect of epigenetic factors on differentiations of pancreatic progenitor cells into insulin-producings cells The effect of epigenetic factors on differentiations of pancreatic progenitor cells into insulin-producings cells
skos:notation
RIV/00023001:_____/11:00002530!RIV12-MZ0-00023001
n9:predkladatel
n17:ico%3A00023001
n3:aktivita
n15:V
n3:aktivity
V
n3:cisloPeriodika
9
n3:dodaniDat
n12:2012
n3:domaciTvurceVysledku
n8:2072777 n8:4620747 n8:7380003 n8:2898616 n8:2888866 n8:1618954
n3:druhVysledku
n18:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
196256
n3:idVysledku
RIV/00023001:_____/11:00002530
n3:jazykVysledku
n4:eng
n3:klicovaSlova
diabetology
n3:klicoveSlovo
n16:diabetology
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[DF224B42E8F2]
n3:nazevZdroje
Transplantation Proceedings
n3:obor
n5:FB
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n12:2011
n3:svazekPeriodika
43
n3:tvurceVysledku
Zacharovová, Klára Pektorová, Lenka Leontovyč, Ivan Koblas, Tomáš Saudek, František Berková, Zuzana
n3:wos
000297485500017
s:issn
0041-1345
s:numberOfPages
5
n10:doi
10.1016/j.transproceed.2011.10.025