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Statements

Subject Item
n2:RIV%2F00023001%3A_____%2F11%3A00002482%21RIV12-MZ0-00023001
rdf:type
n15:Vysledek skos:Concept
dcterms:description
Macrophages located in airways and the alveolar space are continually exposed to different signals from the respiratory mucosa. In this respect, epithelial cells represent an important source of cytokines and mediators modulating the state of activation and/or differentiation of mononuclear phagocytes. Many of the proinflammatory genes induced in macrophages during immune and immunopathological reactions are regulated by transcription factor NF kappa B. The aim of our study was to characterize changes in the expression of genes associated with NF kappa B activation and signalling in THP-1 human macrophages co-cultured with A549 respiratory epithelial cells. At least 4-fold upregulation of mRNA level was found in 29 of 84 tested genes including genes for multiple cytokines and chemokines, membrane antigens and receptors, and molecules associated with NF kappa B signalling. The mRNA induction was confirmed at the level of protein expression by evaluating the release of IL-6 and IL-8 and by ICAM-1 expression. Blocking of one NF kappa B subunit by p65 siRNA inhibited the production of IL-6 in both cell types while IL-8 release from THP-1 cells did not seem to be affected. Macrophages located in airways and the alveolar space are continually exposed to different signals from the respiratory mucosa. In this respect, epithelial cells represent an important source of cytokines and mediators modulating the state of activation and/or differentiation of mononuclear phagocytes. Many of the proinflammatory genes induced in macrophages during immune and immunopathological reactions are regulated by transcription factor NF kappa B. The aim of our study was to characterize changes in the expression of genes associated with NF kappa B activation and signalling in THP-1 human macrophages co-cultured with A549 respiratory epithelial cells. At least 4-fold upregulation of mRNA level was found in 29 of 84 tested genes including genes for multiple cytokines and chemokines, membrane antigens and receptors, and molecules associated with NF kappa B signalling. The mRNA induction was confirmed at the level of protein expression by evaluating the release of IL-6 and IL-8 and by ICAM-1 expression. Blocking of one NF kappa B subunit by p65 siRNA inhibited the production of IL-6 in both cell types while IL-8 release from THP-1 cells did not seem to be affected.
dcterms:title
Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages
skos:prefLabel
Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages
skos:notation
RIV/00023001:_____/11:00002482!RIV12-MZ0-00023001
n15:predkladatel
n17:ico%3A00023001
n3:aktivita
n4:Z n4:P
n3:aktivity
P(ME 906), P(NS10524), Z(MZ0IKEM2005)
n3:cisloPeriodika
10
n3:dodaniDat
n10:2012
n3:domaciTvurceVysledku
n7:1786385 n7:1120654 n7:3456013 n7:9859128 n7:7194641 n7:1246291 n7:8498598
n3:druhVysledku
n9:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
197848
n3:idVysledku
RIV/00023001:_____/11:00002482
n3:jazykVysledku
n12:eng
n3:klicovaSlova
Co-cultures; Cytokines; Epithelial cells; Macrophages; NF kappa B
n3:klicoveSlovo
n6:Co-cultures n6:NF%20kappa%20B n6:Macrophages n6:Cytokines n6:Epithelial%20cells
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[E0930D13854D]
n3:nazevZdroje
Immunobiology
n3:obor
n14:EC
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
10
n3:projekt
n13:ME%20906 n13:NS10524
n3:rokUplatneniVysledku
n10:2011
n3:svazekPeriodika
216
n3:tvurceVysledku
Jarešová, Marcela Brabcová, Irena Slavčev, Antonij Poole, J. A. Kolesár, Libor Stříž, Ilja Brabcová, Eva Liu, X D. Rennard, S. I. Sekerková, Alena
n3:wos
000296365700006
n3:zamer
n16:MZ0IKEM2005
s:issn
0171-2985
s:numberOfPages
7
n20:doi
10.1016/j.imbio.2011.04.009