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Statements

Subject Item
n2:RIV%2F00020702%3A_____%2F14%3A%230000817%21RIV15-MZE-00020702
rdf:type
n13:Vysledek skos:Concept
rdfs:seeAlso
http://www.agriculturejournals.cz/publicFiles/112132.pdf
dcterms:description
Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg.l-1 of glutamine, 200 mg.l-1 of casein hydrolysate, 30 g.l-1 of sucrose, 0.2 mg.l-1 of BAP, and o.1 mg.l-1 of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg.l-1 of IBA 70-75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J. cyanoides. Protocols for the in vitro propagation of two endangered plant species in the Czech Republic, Gentiana verna L. and Jurinea cyanoides (L.) Rchb., were established. In G. verna, the induction of organogenesis on vegetative shoots was successful on the basal 6% agar WPM medium with 200 mg.l-1 of glutamine, 200 mg.l-1 of casein hydrolysate, 30 g.l-1 of sucrose, 0.2 mg.l-1 of BAP, and o.1 mg.l-1 of IBA. The multiplication of primary explants was achieved on 6% agar basal MS medium with the same concentrations of substances mentioned above. In J. cyanoides, the induction of organogenesis and multiplication of its vegetative shoots were successful on MS medium with the same concentration of added substances used in G. verna. On the basal MS medium of 1/3 concentration with 3 mg.l-1 of IBA 70-75% rooting efficiency of G. verna microcuttings and 40% rooting efficiency of J. cyanoides microcuttings were reached. The mortality during acclimatization did not exceed 20% for G. verna and 25% for J. cyanoides.
dcterms:title
Protocol for efficient micropropagation of spring gentian and sand jurinea Protocol for efficient micropropagation of spring gentian and sand jurinea
skos:prefLabel
Protocol for efficient micropropagation of spring gentian and sand jurinea Protocol for efficient micropropagation of spring gentian and sand jurinea
skos:notation
RIV/00020702:_____/14:#0000817!RIV15-MZE-00020702
n3:aktivita
n16:Z
n3:aktivity
Z(MZE0002070203)
n3:cisloPeriodika
1
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n6:6587232 n6:8399239 n6:8290695 n6:8154570
n3:druhVysledku
n5:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
40514
n3:idVysledku
RIV/00020702:_____/14:#0000817
n3:jazykVysledku
n4:eng
n3:klicovaSlova
Gentiana verna; Jurinea cyanoides; in vitro reproduction; organogenesis
n3:klicoveSlovo
n14:Gentiana%20verna n14:in%20vitro%20reproduction n14:Jurinea%20cyanoides n14:organogenesis
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[38B4BCF2DCAA]
n3:nazevZdroje
Journal of Forest Science
n3:obor
n18:EF
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n10:2014
n3:svazekPeriodika
60
n3:tvurceVysledku
Malá, Jana Máchová, Pavlína Cvrčková, Helena Dostál, Jaroslav
n3:zamer
n8:MZE0002070203
s:issn
1212-4834
s:numberOfPages
5