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Statements

Subject Item
n2:RIV%2F00020702%3A_____%2F08%3A9P000718%21RIV09-MZE-00020702
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Cílem práce bylo ověřit, zda filtrát sekundárně patogenní houby Ascocalyx abietina, indukuje u embryogenních kultur smrku ztepilého změny v obsahu fenolických látek , což by bylo indikátorem obranné reakce na napadení patogenem. Zaměřili jsme se na akumulaci rozpustných a stěnově vázaných fenolických látek, stilbenů a na aktivitu extracelulární peroxidázy. Změny vyvolané filtrátem se projevili významně ve zvýšení stěnově vázaných fenolických kyselin (o 130 %) a rozpustných fenolických glykosidů až o 65%.Vliv filtrátu se neprojevil významně v aktivitě extracelulární peroxidázy.Z výsledků vyplývá, že filtrát houby Ascocalyx abietina vyvolává totožné změny v sekundárním metabolismu smrkových buněk jako houbová infekce. The aim of this work was to identify whether the Ascocalyx abietina culture filtrate has the ability to induce changes in the contents of phenolic substances that might be indicative for the disease response of Norway spruce (Picea abies). We focused on the accumulation of soluble and cell wall-bound phenolics, stilbenes as well as extracellular peroxidase activities elicited in the embryogenic cell cultures of Norway spruce by A. abietina culture filtrate. Treatment of spruce cells with fungal culture filtrate (5 and 20% v/v concentrations) evoked an increase in the total contents of phenolic acids (represented by the sum of free, methanol soluble ester- and glycoside-bound phenolics and methanol insoluble cell-wall bound phenolic esters). The challenge with filtrate was in particular manifested in the increase (compared with the control) in the contents of cell wall-bound phenolic acids (by about 100 and 130% in 5 and 20% filtrate, respectively) and soluble phenolic glycosides (by 37 and 65% in 5 an The aim of this work was to identify whether the Ascocalyx abietina culture filtrate has the ability to induce changes in the contents of phenolic substances that might be indicative for the disease response of Norway spruce (Picea abies). We focused on the accumulation of soluble and cell wall-bound phenolics, stilbenes as well as extracellular peroxidase activities elicited in the embryogenic cell cultures of Norway spruce by A. abietina culture filtrate. Treatment of spruce cells with fungal culture filtrate (5 and 20% v/v concentrations) evoked an increase in the total contents of phenolic acids (represented by the sum of free, methanol soluble ester- and glycoside-bound phenolics and methanol insoluble cell-wall bound phenolic esters). The challenge with filtrate was in particular manifested in the increase (compared with the control) in the contents of cell wall-bound phenolic acids (by about 100 and 130% in 5 and 20% filtrate, respectively) and soluble phenolic glycosides (by 37 and 65% in 5 an
dcterms:title
Induced changes in phenolic acids and stilbenes in embryogenic cell cultures of Norway spruce by culture filtrate of Ascocalyx abietina Induced changes in phenolic acids and stilbenes in embryogenic cell cultures of Norway spruce by culture filtrate of Ascocalyx abietina Indukce změn fenolických kyselin a stilbenů u embryogenní kultury smrku ztepilého vyvolané filtrátem Ascocalyx abietina
skos:prefLabel
Induced changes in phenolic acids and stilbenes in embryogenic cell cultures of Norway spruce by culture filtrate of Ascocalyx abietina Indukce změn fenolických kyselin a stilbenů u embryogenní kultury smrku ztepilého vyvolané filtrátem Ascocalyx abietina Induced changes in phenolic acids and stilbenes in embryogenic cell cultures of Norway spruce by culture filtrate of Ascocalyx abietina
skos:notation
RIV/00020702:_____/08:9P000718!RIV09-MZE-00020702
n4:aktivita
n8:Z
n4:aktivity
Z(MZE0002070202)
n4:cisloPeriodika
2
n4:dodaniDat
n13:2009
n4:domaciTvurceVysledku
n12:6587232
n4:druhVysledku
n16:J
n4:duvernostUdaju
n10:S
n4:entitaPredkladatele
n9:predkladatel
n4:idSjednocenehoVysledku
371926
n4:idVysledku
RIV/00020702:_____/08:9P000718
n4:jazykVysledku
n14:eng
n4:klicovaSlova
astringin; isorhapontin; Picea abies; piceid; soluble and cell wall-bound phenolics
n4:klicoveSlovo
n5:soluble%20and%20cell%20wall-bound%20phenolics n5:Picea%20abies n5:isorhapontin n5:piceid n5:astringin
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[307BD6695CD0]
n4:nazevZdroje
Journal of Plant Diseases Protection
n4:obor
n17:GK
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
5
n4:rokUplatneniVysledku
n13:2008
n4:svazekPeriodika
115
n4:tvurceVysledku
Hrubcová, M. Malá, Jana Cvikrová, M. Eder, J. Foretová, S.
n4:wos
000255261900002
n4:zamer
n11:MZE0002070202
s:issn
1861-4051
s:numberOfPages
6