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Statements

Subject Item
n2:RIV%2F00000493%3AG41__%2F11%3A%230000388%21RIV12-MO0-G41_____
rdf:type
skos:Concept n14:Vysledek
dcterms:description
The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5α in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions. The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5α in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.
dcterms:title
Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols
skos:prefLabel
Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols
skos:notation
RIV/00000493:G41__/11:#0000388!RIV12-MO0-G41_____
n14:predkladatel
n17:orjk%3AG41
n4:aktivita
n18:P
n4:aktivity
P(OVVTUO2008001)
n4:cisloPeriodika
3
n4:dodaniDat
n8:2012
n4:domaciTvurceVysledku
n5:6669719 n5:8272433
n4:druhVysledku
n16:J
n4:duvernostUdaju
n6:S
n4:entitaPredkladatele
n19:predkladatel
n4:idSjednocenehoVysledku
197034
n4:idVysledku
RIV/00000493:G41__/11:#0000388
n4:jazykVysledku
n13:eng
n4:klicovaSlova
Bioagents; Sandwich immunoassay; Biosensors; Remote sensing; Aerosol chamber
n4:klicoveSlovo
n7:Biosensors n7:Sandwich%20immunoassay n7:Aerosol%20chamber n7:Remote%20sensing n7:Bioagents
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[1295AE0E9F10]
n4:nazevZdroje
Electroanalysis
n4:obor
n10:CE
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
7
n4:projekt
n15:OVVTUO2008001
n4:rokUplatneniVysledku
n8:2011
n4:svazekPeriodika
24
n4:tvurceVysledku
Švábenská, Eva Skládal, Petr
s:issn
1040-0397
s:numberOfPages
8
n12:organizacniJednotka
G41