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Statements

Subject Item
n2:7E11011
rdf:type
n7:Projekt
rdfs:seeAlso
http://www.isvav.cz/projectDetail.do?rowId=7E11011
dcterms:description
This project aims to develop novel biocatalysts for the production of glycosides (NOVOSIDES). Glycosylated compounds have a wide range of applications, but very few enzymes are able to glycosylate small organic molecules cost-efficiently at the industrial scale. Therefore, glycosylation reactions catalysed by transglycosidases, glycoside phosphorylases and glycoside hydrolases will be explored in more detail. These enzymes catalyze the transfer a glycosyl group from a cheap and readily available donor substrate to a variety of acceptors. To exploit their full potential, the enzymes’ specificity and stability against high temperatures and the presence of organic co-solvents will be optimised by means of directed evolution. This project aims to develop novel biocatalysts for the production of glycosides (NOVOSIDES). Glycosylated compounds have a wide range of applications, but very few enzymes are able to glycosylate small organic molecules cost-efficiently at the industrial scale. Therefore, glycosylation reactions catalysed by transglycosidases, glycoside phosphorylases and glycoside hydrolases will be explored in more detail. These enzymes catalyze the transfer a glycosyl group from a cheap and readily available donor substrate to a variety of acceptors. To exploit their full potential, the enzymes’ specificity and stability against high temperatures and the presence of organic co-solvents will be optimised by means of directed evolution.
dcterms:title
Novel Biocatalysts for the Production of Glycosides Novel Biocatalysts for the Production of Glycosides
skos:notation
7E11011
n3:aktivita
n9:7E
n3:celkovaStatniPodpora
n6:celkovaStatniPodpora
n3:celkoveNaklady
n6:celkoveNaklady
n3:datumDodatniDoRIV
2015-02-16+01:00
n3:druhSouteze
n19:RP
n3:duvernostUdaju
n17:S
n3:fazeProjektu
n14:100525992
n3:hlavniObor
n8:CE
n3:kategorie
n15:ZV
n3:klicovaSlova
Glycoside; transglycosidase; phosphorylase; directed evolution; metagenome; fluorescence probes
n3:partnetrHlavni
n12:ico%3A61388971
n3:pocetKoordinujicichPrijemcu
0
n3:pocetPrijemcu
1
n3:pocetSpoluPrijemcu
0
n3:pocetVysledkuRIV
13
n3:pocetZverejnenychVysledkuVRIV
13
n3:posledniUvolneniVMinulemRoce
2013-03-27+01:00
n3:prideleniPodpory
n10:MSMT-1839%2F2013-310
n3:sberDatUcastniciPoslednihoRoku
n4:2014
n3:sberDatUdajeProjZameru
n4:2014
n3:statusZobrazovaneFaze
n18:DRRVK
n3:typPojektu
n11:P
n3:ukonceniReseni
2014-11-30+01:00
n3:vedlejsiObor
n8:EI n8:EE
n3:zahajeniReseni
2011-01-01+01:00
n3:zivotniCyklusProjektu
n13:ZBBK
n3:vyzva
n20:FP7-KBBE-2010-4
n3:klicoveSlovo
phosphorylase Glycoside metagenome directed evolution transglycosidase