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Statements

Subject Item
n2:1629
rdf:type
n4:Projekt
dcterms:description
Infection of wheat by fungus B. graminis is causing powdery mildew disease. Recently, non-race-specific powdery mildew resistance gene QPm.tut-4A was mapped on distal end of long arm of wheat chromosome 4A. Here we propose to construct two BAC DNA libraries specific for 4AL chromosomal arm. The huge genome and polyploid character hamper positional cloning in wheat. The proposed libraries would become keys for solving the obstruction and stepping stones for creation of physical map of the 4A chromosome. The physical map will facilitate cloning of important genes from the chromosome, including resistance genes to powdery mildew, leaf rust, Hessian fly, yellow rust, stem rust, wheat streak mosaic and Septoria tritici blotch. The main objective of the project is cloning of the powdery mildew resistance gene QPm.tut-4A. High density map of the QPm.tut-4A gene region will be created and BAC contig spanning the region identified. Subsequently, a pooled genomic BAC library will be prepared from the donor of resistance, T. militinae, and will be used to clone the QPm.tut-4A gene. Cílem navrhovaného projektu je konstrukce BAC DNA knihoven z dlouhého ramene pšeničného chromosomu 4A pro přípravu vysoko-hustotní a fyzické mapy regionu genu QPm.tut-4A odpovědného za zvýšenou rezistenci k padlí travní a klonování tohoto genu.
dcterms:title
Konstrukce BAC knihoven specifických pro chromosom 4AL a poziční klonování genu pro rezistenci dospělých rostlin k padlí travní u pšenice Construction of BAC DNA libraries specific for chromosome 4AL, and positional cloning of gene for adult plant resistance to powdery mildew in wheat
n4:cislo-smlouvy
n11:1629
n4:dalsi-vedlejsi-obor
n9:EB
n4:druh-souteze
n8:VS
n4:faze
n5:52828207
n4:hlavni-obor
n9:GE
n4:vedlejsi-obor
n9:GF
n4:id-aktivity
n7:GA
n4:id-souteze
n12:SGA02008GA-ST
n4:kategorie
n6:1
n4:klicova-slova
Triticum aestivum; BAC; chromozómově specifické knihovny, poziční klonování; padlí travní
n4:konec-reseni
2011-12-31+01:00
n4:pocet-koordinujicich-prijemcu
0
n4:poskytovatel
n10:GA0
n4:start-reseni
2008-01-01+01:00
n4:statni-podpora
3959
n4:typProjektu
n13:P
n4:uznane-naklady
3959
n4:pocet-prijemcu
1
n4:pocet-spoluprijemcu
1
n4:pocet-vysledku
2
n4:pocet-vysledku-zverejnovanych
2