About: Yeast One/Two-Hybrid System     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : owl:Class, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
rdfs:label
  • Yeast One/Two-Hybrid System
rdfs:subClassOf
Semantic_Type
  • Molecular Biology Research Technique
Preferred_Name
  • Yeast One/Two-Hybrid System
UMLS_CUI
  • C1961118
ALT_DEFINITION
  • two hybrid proteins interact to transcriptionally activate a reporter gene in vivo.CSP2000
Legacy_Concept_Name
  • Yeast_One_Two-Hybrid_System
FULL_SYN
  • Yeast One Hybrid SystemSYNCI
  • Yeast Two Hybrid SystemSYNCI
  • Yeast One/Two-Hybrid SystemPTNCI
  • Two HybridSYNCI
DEFINITION
  • Molecular biology technique used to identify interacting proteins in a yeast model. A protein of interest (the bait) is fused to the DNA-binding domain of a yeast transcription factor (usually GAL4). (Usually) a cDNA library is fused to the GAL4 activation domain. The library is then introduced into yeast cells carrying the bait construct. Functional interaction of the bait with fusion proteins from the library bring together the binding and activation domains of the transcription factor, allowing activation of a reporter gene. (syn: yeast two-hybrid system) (NCI/OSP)NCI
code
  • C18140
http://linked.open...y/mesh/hasConcept
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