About: Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma

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About: Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Histologically verified pairs (n=10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw C{q} values correlated with the degree of RNA degradation. This correlation was abolished by normalization to C{q} of 18S endogenous control gene. Both geNorm and NormFinder programs suggested EIF2B1, ELF1, MRPL19, and POP4 as the same most stable genes. We have thus identified suitable reference genes for future expression studies in pancreatic carcinoma. Normalization method reducing the effects of RNA degradation on the quality of results was also developed. Histologically verified pairs (n=10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw C{q} values correlated with the degree of RNA degradation. This correlation was abolished by normalization to C{q} of 18S endogenous control gene. Both geNorm and NormFinder programs suggested EIF2B1, ELF1, MRPL19, and POP4 as the same most stable genes. We have thus identified suitable reference genes for future expression studies in pancreatic carcinoma. Normalization method reducing the effects of RNA degradation on the quality of results was also developed. (en)
Title	Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma (en)
skos:prefLabel	Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma (en)
skos:notation	RIV/75010330:_____/12:00009617!RIV13-MZ0-75010330
http://linked.open...avai/predkladatel	Státní zdravotní ústav
http://linked.open...avai/riv/aktivita	P V
http://linked.open...avai/riv/aktivity	P(NS10230), P(NS9799), V
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Souček, Pavel Mohelníková-Duchoňová, Beatrice
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Státní zdravotní ústav, příspěvková organizace
http://linked.open...dnocenehoVysledku	135161
http://linked.open...ai/riv/idVysledku	RIV/75010330:_____/12:00009617
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	pancreas carcinoma; pancreas; carcinoma; PCR; RNA (en)
http://linked.open.../riv/klicoveSlovo	carcinoma PCR RNA pancreas carcinoma pancreas
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[484F2B721EC6]
http://linked.open...i/riv/nazevZdroje	Disease markers
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of relation between expression profiles in tumor tissue and result of chemotherapy of breast carcinomas Rationalization of strategy of oncological treatment of radically inoperable colorectal liver metastases
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	32
http://linked.open...iv/tvurceVysledku	Souček, Pavel Honsová, E. Mohelníková-Duchoňová, Beatrice Oliverius, M.
http://linked.open...ain/vavai/riv/wos	000301045600008
issn	0278-0240
number of pages	8 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Evaluation of reference genes and normalization strategy for quantitative real-time PCR in human pancreatic carcinoma

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