About: Efect of organic acids on bacterium campylobacter jejuni

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of the Doctoral thesis was to evaluate the antimicrobial activity of nineteen organic acids and two monoacylglycerols against Campylobacter jejuni. The objective was to determine inhibitory concentrations of organic acids in vitro and to confirm these results also by in vivo experiment. Two different methods were used to assess the antimicrobial activity of acids: the measurement of cell protein concentration in treated cultures by the biuret method and a SYBR Green based real-time PCR assay. Caprylic, capric and lauric acids were the most efficient antimicrobials among the compounds tested (IC50 <= 0.10 mg/ml). Antimicrobial acitivity of organic acids was pH-dependent, being more pronounced at a lower pH than at a pH higher than 6. The effect of the time of incubation was less evident. Clinical isolate of C. jejuni showed higher tolerance to exposure of organic acids compare to CCM 6214T strain. In the cells treated with capric acid, fumaric acid and monocaprin, the permeability of the c The aim of the Doctoral thesis was to evaluate the antimicrobial activity of nineteen organic acids and two monoacylglycerols against Campylobacter jejuni. The objective was to determine inhibitory concentrations of organic acids in vitro and to confirm these results also by in vivo experiment. Two different methods were used to assess the antimicrobial activity of acids: the measurement of cell protein concentration in treated cultures by the biuret method and a SYBR Green based real-time PCR assay. Caprylic, capric and lauric acids were the most efficient antimicrobials among the compounds tested (IC50 <= 0.10 mg/ml). Antimicrobial acitivity of organic acids was pH-dependent, being more pronounced at a lower pH than at a pH higher than 6. The effect of the time of incubation was less evident. Clinical isolate of C. jejuni showed higher tolerance to exposure of organic acids compare to CCM 6214T strain. In the cells treated with capric acid, fumaric acid and monocaprin, the permeability of the c (en)
Title	Efect of organic acids on bacterium campylobacter jejuni Efect of organic acids on bacterium campylobacter jejuni (en)
skos:prefLabel	Efect of organic acids on bacterium campylobacter jejuni Efect of organic acids on bacterium campylobacter jejuni (en)
skos:notation	RIV/70883521:28110/09:63508251!RIV10-MSM-28110___
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Molatová, Zuzana
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Tomáše Bati ve Zlíně / Fakulta technologická
http://linked.open...dnocenehoVysledku	311942
http://linked.open...ai/riv/idVysledku	RIV/70883521:28110/09:63508251
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Campylobacter jejuni; organic acids; antimicrobial effect; inhibitory concentration; real-time PCR; replacements of antibiotics (en)
http://linked.open.../riv/klicoveSlovo	antimicrobial effect organic acids Campylobacter jejuni real-time PCR inhibitory concentration replacements of antibiotics
http://linked.open...ontrolniKodProRIV	[8F883BFE1BEA]
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Molatová, Zuzana
http://localhost/t...ganizacniJednotka	28110

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