About: Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting     Goto   Sponge   NotDistinct   Permalink

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Description
  • Byl ověřován postup isolace bakteriální DNA z velmi humosních stepních půd. Huminové látky ztěžovaly isolaci DNA z půd i inhibovaly následnou proceduru PCR. Práce prokázala, že dodatečné přečištění získané DNA je nezbytné. Z některých typů vzorků je však nemožné získat požadovanou DNA universálním postupem. (cs)
  • In our study we aimed to find the universal procedure which would enable us to get DGGE profiles from larger set of samples where the soils differed substantially in contents of organic matter. Fraction of humic substances displays similar properties to DNA hence it can be uneasy to remove them during DNA isolation process and the substances consequently inhibit PCR. Additional cleaning of isolated DNA appears to be necessary. for most of our samples, two cleanings of isolated DNA (Wizard DNA clean-up systém, Promega) and subsequent tenfold dilution were sufficient. The most problematic sample needed three cleaning steps and tenfold dilution. Our experiences imply that dilution is more useful than additional cleaning for some samples.
  • In our study we aimed to find the universal procedure which would enable us to get DGGE profiles from larger set of samples where the soils differed substantially in contents of organic matter. Fraction of humic substances displays similar properties to DNA hence it can be uneasy to remove them during DNA isolation process and the substances consequently inhibit PCR. Additional cleaning of isolated DNA appears to be necessary. for most of our samples, two cleanings of isolated DNA (Wizard DNA clean-up systém, Promega) and subsequent tenfold dilution were sufficient. The most problematic sample needed three cleaning steps and tenfold dilution. Our experiences imply that dilution is more useful than additional cleaning for some samples. (en)
Title
  • Isolace bakteriální DNA z velmi humosních stepních půd a následná DGGE Fingerprinting (cs)
  • Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting
  • Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting (en)
skos:prefLabel
  • Isolace bakteriální DNA z velmi humosních stepních půd a následná DGGE Fingerprinting (cs)
  • Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting
  • Isolation of Bacterial DNA from very Humic Steppe Soils and Subsequent DGGE Fingerprinting (en)
skos:notation
  • RIV/70883521:28110/04:63502562!RIV/2005/MSM/281105/N
http://linked.open.../vavai/riv/strany
  • 120-123
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 281100002)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 569074
http://linked.open...ai/riv/idVysledku
  • RIV/70883521:28110/04:63502562
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • bacterial DNA; isolation; soil; humic (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [0C07C873DCB5]
http://linked.open...v/mistoKonaniAkce
  • České Budějovice
http://linked.open...i/riv/mistoVydani
  • České Budějovice
http://linked.open...i/riv/nazevZdroje
  • 9th Methodological workshop: Present Methods for Investigation of Microbial Community Biodiversity in Soils and Substrates
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Koutný, Marek
  • Braster, M.
  • van Verseveld, H. W.
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Ústav půdní biologie AV ČR
http://localhost/t...ganizacniJednotka
  • 28110
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