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  • We have used several visualization techniques to characterize biological objects. A micro-radiography with the hybrid single photon counting silicon pixel detector Medipix2 (matrix 256 x 256 sq. pixels of 55 μm pitch) is an imaging technique using X-rays in the studies of internal structures of objects. The detector Medipix2 is used as an imager of an ionizing radiation, emitted by X-ray tubes (micro or nano-focus FeinFocus). An unlimited dynamic range of the Medipix2 detector and a high spatial resolution below 1 μm is particularly suitable for a non-destructive and non-invasive radiographic imaging of small biological samples in a living state, including in vivo observations and a micro-tomography. Contrast agents (based on iodine or lanthanum) could be used for dynamic studies inside of organisms. Infrared digital photography has ability to shot still photographs or movies in complete dark. Is it also possible to use it for studies of internal organs and structures inside of living biological objects. Field emission scanning electron microscopy (FESEM) in low temperature mode is sophisticated recent technique successfully used in biological laboratories. The main advantage is ability to study details of tissues and cells close to living state at very high magnification. Special cryotransfer system connected to FESEM allows deeply frozen samples to be prepared in way like freeze-fracturing followed by freeze-etching for observation directly inside of electron microscope. Combination of information from all above mentioned techniques could give us very powerful visualization tool for complex studies of biological specimen.
  • We have used several visualization techniques to characterize biological objects. A micro-radiography with the hybrid single photon counting silicon pixel detector Medipix2 (matrix 256 x 256 sq. pixels of 55 μm pitch) is an imaging technique using X-rays in the studies of internal structures of objects. The detector Medipix2 is used as an imager of an ionizing radiation, emitted by X-ray tubes (micro or nano-focus FeinFocus). An unlimited dynamic range of the Medipix2 detector and a high spatial resolution below 1 μm is particularly suitable for a non-destructive and non-invasive radiographic imaging of small biological samples in a living state, including in vivo observations and a micro-tomography. Contrast agents (based on iodine or lanthanum) could be used for dynamic studies inside of organisms. Infrared digital photography has ability to shot still photographs or movies in complete dark. Is it also possible to use it for studies of internal organs and structures inside of living biological objects. Field emission scanning electron microscopy (FESEM) in low temperature mode is sophisticated recent technique successfully used in biological laboratories. The main advantage is ability to study details of tissues and cells close to living state at very high magnification. Special cryotransfer system connected to FESEM allows deeply frozen samples to be prepared in way like freeze-fracturing followed by freeze-etching for observation directly inside of electron microscope. Combination of information from all above mentioned techniques could give us very powerful visualization tool for complex studies of biological specimen. (en)
Title
  • Combination of modern visualization techniques for imaging of biological samples
  • Combination of modern visualization techniques for imaging of biological samples (en)
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  • Combination of modern visualization techniques for imaging of biological samples
  • Combination of modern visualization techniques for imaging of biological samples (en)
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  • RIV/68407700:21670/12:00222752!RIV15-MSM-21670___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(LA08015), Z(MSM6840770040)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
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  • 127676
http://linked.open...ai/riv/idVysledku
  • RIV/68407700:21670/12:00222752
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http://linked.open.../riv/klicovaSlova
  • silicon pixel detector; Medipix2; visualization techniques; Field Emission Scanning Electron Microscopy (FESEM); radiographic imaging (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [D8FD11E8F519]
http://linked.open...v/mistoKonaniAkce
  • Como (Villa Olmo)
http://linked.open...i/riv/mistoVydani
  • Singapore
http://linked.open...i/riv/nazevZdroje
  • %22Astroparticle, Particle, Space Physics and Detectors for Physics Applications%22, Proceedings of the 13th ICATPP Conference, Villa Olmo, Como, Italy, 3-7 October 2011
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Dammer, Jiří
  • Weyda, F.
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://bibframe.org/vocab/doi
  • 10.1142/9789814405072_0107
http://purl.org/ne...btex#hasPublisher
  • World Scientific Publishing, Ltd.
https://schema.org/isbn
  • 978-981-4405-06-5
http://localhost/t...ganizacniJednotka
  • 21670
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