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  • The aim of this work was to investigate mutations associated with cardiovascular diseases using polymerase chain reaction (PCR) with reverse hybridization; and to evaluate the implication of the results on cardiovascular disease susceptibility. The procedure involved DNA isolation, PCR amplification using biotinylated primers and hybridizing amplification products to test strips containing immobilized allele specific oligonucleotide probes. Blood specimens from 18 dyslipidemic patients and 16 healthy controls were used. Eight genes and their variants (eNOS-786T-C and G894T; LTA C804A; ACE ins/del; GP IIIa (HPA1) a/b; beta-FBG-455G-A; ApoB R3500Q; APOE E2/E3/E4) were detected. Data analysis was done using SPSS for Windows. ENOS-786T-C homozygous state was found only in patients, GPIIIa (a/b) was more in patients (85.71 %) than in controls (14.29 %); only wild type ApoB R3500Q was found in the controls while patients showed bothwild type and heterozygous forms. The heterozygous form of ApoB R3500Q showed a hereditary trait. It also appears to be the strongest independent risk factor for dyslipidemia and cardiovascular diseases. There seems to be a strong correlation between dyslipidemia and GPIIIa and ApoB mutations.
  • The aim of this work was to investigate mutations associated with cardiovascular diseases using polymerase chain reaction (PCR) with reverse hybridization; and to evaluate the implication of the results on cardiovascular disease susceptibility. The procedure involved DNA isolation, PCR amplification using biotinylated primers and hybridizing amplification products to test strips containing immobilized allele specific oligonucleotide probes. Blood specimens from 18 dyslipidemic patients and 16 healthy controls were used. Eight genes and their variants (eNOS-786T-C and G894T; LTA C804A; ACE ins/del; GP IIIa (HPA1) a/b; beta-FBG-455G-A; ApoB R3500Q; APOE E2/E3/E4) were detected. Data analysis was done using SPSS for Windows. ENOS-786T-C homozygous state was found only in patients, GPIIIa (a/b) was more in patients (85.71 %) than in controls (14.29 %); only wild type ApoB R3500Q was found in the controls while patients showed bothwild type and heterozygous forms. The heterozygous form of ApoB R3500Q showed a hereditary trait. It also appears to be the strongest independent risk factor for dyslipidemia and cardiovascular diseases. There seems to be a strong correlation between dyslipidemia and GPIIIa and ApoB mutations. (en)
Title
  • Technologies In Molecular Biology for Genomics and Personalized Medicine Focused on PCR with Reverse Hybridization
  • Technologies In Molecular Biology for Genomics and Personalized Medicine Focused on PCR with Reverse Hybridization (en)
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  • Technologies In Molecular Biology for Genomics and Personalized Medicine Focused on PCR with Reverse Hybridization
  • Technologies In Molecular Biology for Genomics and Personalized Medicine Focused on PCR with Reverse Hybridization (en)
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  • RIV/68407700:21460/12:00202763!RIV13-MSM-21460___
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  • P(EE.2.3.20.0092)
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  • 173603
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  • RIV/68407700:21460/12:00202763
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  • cardiovascular diseases; DNA isolation (en)
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  • [46DB45E93909]
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  • Kladno
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  • Prague
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  • Instuments and Methods for Biology and Medicine 2012
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  • Jarošíková, Taťána
  • Kotaška, K.
  • Shivairo, Daniel Swegenyi Kivairo
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  • České vysoké učení technické v Praze
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  • 978-80-01-05119-1
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  • 21460
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