About: Using genetic engineering methods for detection lower fungi in digestive tract of primates

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this work is detection of eukaryotic organisms-fungi-in digestive tract of primates. The presence of fungi in digestive tract of primates has not been studied yet. The first part of project is to establish appropriate methods of genetic engineering and optimize these methods. Control samples of mouflon faeces are used to establish methodology, because mouflon digestive tract contains fungi, so results of our testing experiments for fungal detection should come outpositive. DNA is isolated from control samples of mouflon faeces, products of DNA amplification by PCR are analyzed by agarose gel electrophoresis.The main goal is setting PCR condition to increase sensitivity of detection of fungal ITS regions. The next step is sequencing and comparison of sequences in the nucleotide database to find typesof fungi. Final testing and experiments at gorilla faeces will take place in future. The aim of this work is detection of eukaryotic organisms-fungi-in digestive tract of primates. The presence of fungi in digestive tract of primates has not been studied yet. The first part of project is to establish appropriate methods of genetic engineering and optimize these methods. Control samples of mouflon faeces are used to establish methodology, because mouflon digestive tract contains fungi, so results of our testing experiments for fungal detection should come outpositive. DNA is isolated from control samples of mouflon faeces, products of DNA amplification by PCR are analyzed by agarose gel electrophoresis.The main goal is setting PCR condition to increase sensitivity of detection of fungal ITS regions. The next step is sequencing and comparison of sequences in the nucleotide database to find typesof fungi. Final testing and experiments at gorilla faeces will take place in future. (en)
Title	Using genetic engineering methods for detection lower fungi in digestive tract of primates Using genetic engineering methods for detection lower fungi in digestive tract of primates (en)
skos:prefLabel	Using genetic engineering methods for detection lower fungi in digestive tract of primates Using genetic engineering methods for detection lower fungi in digestive tract of primates (en)
skos:notation	RIV/68407700:21460/12:00202752!RIV13-MSM-21460___
http://linked.open...avai/predkladatel	Fakulta biomedicínského inženýrství
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(EE.2.3.20.0092)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Jarošíková, Taťána Hamrová, Jitka
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	České vysoké učení technické v Praze / Fakulta biomedicínského inženýrství
http://linked.open...dnocenehoVysledku	176503
http://linked.open...ai/riv/idVysledku	RIV/68407700:21460/12:00202752
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	eukaryotic organisms-fungi-in; digestive tract; primates; genetic engineering; , DNA; , PCR condition (en)
http://linked.open.../riv/klicoveSlovo	genetic engineering DNA digestive tract primates PCR condition eukaryotic organisms-fungi-in
http://linked.open...ontrolniKodProRIV	[B63A5D383CB4]
http://linked.open...v/mistoKonaniAkce	Kladno
http://linked.open...i/riv/mistoVydani	Prague
http://linked.open...i/riv/nazevZdroje	Instuments and Methods for Biology and Medicine 2012
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	BIO-XUV Research Team Advancement at FBME CTU
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Jarošíková, Taťána Fliegerová, K. Mrázek, J. Hamrová, Jitka
http://linked.open...vavai/riv/typAkce	CST - Celostátní
http://linked.open.../riv/zahajeniAkce	2012-05-29 (xsd:date)
number of pages	3 (xsd:int)
http://purl.org/ne...btex#hasPublisher	České vysoké učení technické v Praze
https://schema.org/isbn	978-80-01-05119-1
http://localhost/t...ganizacniJednotka	21460
is http://linked.open...avai/riv/vysledek of	Using genetic engineering methods for detection lower fungi in digestive tract of primates

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