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  • Testate amoebae (Protozoa:Rhizopoda) are a group of unicellular animals (20 - 400 μm), protected with the shell (SiO2, CaCO3, proteins), living in fresh water habitats. They are used as model organisms in population ecology, ecotoxicology, paleoecology - thanks to their cosmopolitan disperse and species-specific ecological preferences together with low ecological valence to the changes of the environmental surroundings. Testate amoebae are usually examined using scanning electron microscopy (SEM) and environmental SEM (E-SEM). Scanning electron microscope allows for much better resolution than confocal laser scanning microscopy (CLSM) far sufficient for recognizing determinant features), but is limited to viewing surface of the specimen. For this reason, we use CLSM and two-photon excitation (TPE) fluorescence microscopy to visualize the shell and inner structures of testate amoebae. We tried as many as 15 fluorescent dyes.
  • Testate amoebae (Protozoa:Rhizopoda) are a group of unicellular animals (20 - 400 μm), protected with the shell (SiO2, CaCO3, proteins), living in fresh water habitats. They are used as model organisms in population ecology, ecotoxicology, paleoecology - thanks to their cosmopolitan disperse and species-specific ecological preferences together with low ecological valence to the changes of the environmental surroundings. Testate amoebae are usually examined using scanning electron microscopy (SEM) and environmental SEM (E-SEM). Scanning electron microscope allows for much better resolution than confocal laser scanning microscopy (CLSM) far sufficient for recognizing determinant features), but is limited to viewing surface of the specimen. For this reason, we use CLSM and two-photon excitation (TPE) fluorescence microscopy to visualize the shell and inner structures of testate amoebae. We tried as many as 15 fluorescent dyes. (en)
  • Použili jsme laserový konfokální rastrovací mikroskop a dvoufotonovou excitační fluorescenční mikroskopii pro studium schránky a vnitřních struktur Testate Amoebae (krytenek). Použili jsme 15 fluorescenčních barviček. (cs)
Title
  • The Use of Confocal and Two Photon Excitation Microscopy to Study Testate Amoebae
  • The Use of Confocal and Two Photon Excitation Microscopy to Study Testate Amoebae (en)
  • Použití konfokální a dvoufotonové excitační mikroskopie pro studium Testate Amoebae (cs)
skos:prefLabel
  • The Use of Confocal and Two Photon Excitation Microscopy to Study Testate Amoebae
  • The Use of Confocal and Two Photon Excitation Microscopy to Study Testate Amoebae (en)
  • Použití konfokální a dvoufotonové excitační mikroskopie pro studium Testate Amoebae (cs)
skos:notation
  • RIV/68407700:21460/08:12148202!RIV09-MSM-21460___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6840770012)
http://linked.open...iv/cisloPeriodika
  • 4
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http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 401735
http://linked.open...ai/riv/idVysledku
  • RIV/68407700:21460/08:12148202
http://linked.open...riv/jazykVysledku
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  • confocal microscopy; testate amoebae (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [BD1303749F59]
http://linked.open...i/riv/nazevZdroje
  • Physiological Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 53
http://linked.open...iv/tvurceVysledku
  • Čapek, Martin
  • Burdíková, Z.
  • Kubínová, L.
  • Holcová, K.
http://linked.open...n/vavai/riv/zamer
issn
  • 0862-8408
number of pages
http://localhost/t...ganizacniJednotka
  • 21460
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