About: Intensity and Contrast Restoration of Data Sets Captured by a Confocal Microscope

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Vyvinuli jsme metodu pro obnovení jasu a kontrastu dat z konfokálního mikroskopu. Nejdříve se konstruuje standardní histogram celého souboru optických řezů a poté jsou jednotlivé histogramy optických řezů warpovány vzhledem k tomuto histogramu. (cs) We apply a confocal laser scanning microscope (CLSM) to capture a stack of fluorescent optical sections from a biological specimen. However, fluorescent image intensities suffer from light loss distortions showing contrast and brightness degradation of images with depth. This effect causes difficulties in analysis and segmentation of studied objects. Therefore, we developed a method to correct this problem. It is a two step algorithm. In the first step the algorithm determines a standard histogram of the stack of images, and in the second step histograms of individual images are warped according to this standard histogram. The algorithm results in more stable brightness and contrast of images through the stack. We apply a confocal laser scanning microscope (CLSM) to capture a stack of fluorescent optical sections from a biological specimen. However, fluorescent image intensities suffer from light loss distortions showing contrast and brightness degradation of images with depth. This effect causes difficulties in analysis and segmentation of studied objects. Therefore, we developed a method to correct this problem. It is a two step algorithm. In the first step the algorithm determines a standard histogram of the stack of images, and in the second step histograms of individual images are warped according to this standard histogram. The algorithm results in more stable brightness and contrast of images through the stack. (en)
Title	Intensity and Contrast Restoration of Data Sets Captured by a Confocal Microscope Obnovení jasu a kontrastu datových souborů získaných konfokálním mikroskopem (cs) Intensity and Contrast Restoration of Data Sets Captured by a Confocal Microscope (en)
skos:prefLabel	Intensity and Contrast Restoration of Data Sets Captured by a Confocal Microscope Obnovení jasu a kontrastu datových souborů získaných konfokálním mikroskopem (cs) Intensity and Contrast Restoration of Data Sets Captured by a Confocal Microscope (en)
skos:notation	RIV/68407700:21460/05:12108522!RIV09-MSM-21460___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA100110502), P(IAA600110507), P(KJB6011309), Z(AV0Z50110509), Z(MSM6840770012)
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Čapek, Martin Smrčka, Pavel Hána, Karel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	České vysoké učení technické v Praze / Fakulta biomedicínského inženýrství
http://linked.open...dnocenehoVysledku	525271
http://linked.open...ai/riv/idVysledku	RIV/68407700:21460/05:12108522
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	brightness correction; confocal microscopy; contrast correction; histogram warping (en)
http://linked.open.../riv/klicoveSlovo	confocal microscopy brightness correction contrast correction histogram warping
http://linked.open...ontrolniKodProRIV	[53028AA084FF]
http://linked.open...v/mistoKonaniAkce	Praha
http://linked.open...i/riv/mistoVydani	Prague
http://linked.open...i/riv/nazevZdroje	Proceedings of Prague Post Genome Technology Workshop (PPGT 2005)
http://linked.open...in/vavai/riv/obor	JC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	New methods of three-dimensional analysis of anatomical structure of Norway spruce needles using stereology, image analysis ant two-photon microscopy Spatial statistics of 3D confocal images of capillary network Three-dimensional analysis of endoplasmic reticulum structure based on processing of images captured by 2-photon and confocal microscopy
http://linked.open...UplatneniVysledku	2005
http://linked.open...iv/tvurceVysledku	Čapek, Martin Hána, Karel Smrčka, Pavel Kubínová, L.
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2005-06-06 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka Transdisciplinární výzkum v oblasti biomedicínského inženýrství II
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Czech Pattern Recognition Society
https://schema.org/isbn	80-01-03239-6
http://localhost/t...ganizacniJednotka	21460
is http://linked.open...avai/riv/vysledek of	Intensity and Contrast Restoration of Data Sets Captured by a Confocal Microscope

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