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| Description
| - In this study, we determined serum levels of metallothioneins (MTs) and zinc in children with solid tumours (neuroblastoma, Hodgkin lymphoma, medulloblastoma, osteosarcoma, Ewing sarcoma and nephroblastoma) by differential pulse voltammetry Brdicka reaction and enzyme-linked immunosorbent assay. Zn(II) level in patients sera was 40 % compared to controls, contrariwise, MT level was 4.2 x higher in patients. No significant differences among single diagnoses were found both for Zn(II) and MT. When determined Zn(II)/ MT ratio, in controls its value was 24.6, but it was 2.6 in patients. After western-blotting with anti-MT and anti-Zn chicken antibodies, variable intensities of the bands within the samples were observed. The brightness curve obtained for each sample both for MT- and Zn-blots was further analysed to produce a list of band positions together with some complementary information related to the intensity of the observed bands by the optimized algorithm. We constructed from those profiles decision trees that enables distinguish different groups of tumours. Brightness curves of the studied blood samples exhibit interesting similarities. Although they were heat-treated samples, in which we suppose mainly MT, samples contained other thermostable zinc-containing proteins that were helpful for identification of embryonal tumours with 88% accuracy and for identification of sarcomas with 78% accuracy. In MT blots the accuracies were 53% and 45%, respectively. Simultaneous analysis of MT and Zn blots did not increased accuracy of identification neither in embryonal tumours (80%) nor in sarcomas.
- In this study, we determined serum levels of metallothioneins (MTs) and zinc in children with solid tumours (neuroblastoma, Hodgkin lymphoma, medulloblastoma, osteosarcoma, Ewing sarcoma and nephroblastoma) by differential pulse voltammetry Brdicka reaction and enzyme-linked immunosorbent assay. Zn(II) level in patients sera was 40 % compared to controls, contrariwise, MT level was 4.2 x higher in patients. No significant differences among single diagnoses were found both for Zn(II) and MT. When determined Zn(II)/ MT ratio, in controls its value was 24.6, but it was 2.6 in patients. After western-blotting with anti-MT and anti-Zn chicken antibodies, variable intensities of the bands within the samples were observed. The brightness curve obtained for each sample both for MT- and Zn-blots was further analysed to produce a list of band positions together with some complementary information related to the intensity of the observed bands by the optimized algorithm. We constructed from those profiles decision trees that enables distinguish different groups of tumours. Brightness curves of the studied blood samples exhibit interesting similarities. Although they were heat-treated samples, in which we suppose mainly MT, samples contained other thermostable zinc-containing proteins that were helpful for identification of embryonal tumours with 88% accuracy and for identification of sarcomas with 78% accuracy. In MT blots the accuracies were 53% and 45%, respectively. Simultaneous analysis of MT and Zn blots did not increased accuracy of identification neither in embryonal tumours (80%) nor in sarcomas. (en)
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| Title
| - Use of Brightness Wavelet Transformation for Automated Analysis of Serum Metallothioneins and Zinc-Containing-Proteins by Western Blots to Subclassify the Childhood Solid Tumours
- Use of Brightness Wavelet Transformation for Automated Analysis of Serum Metallothioneins and Zinc-Containing-Proteins by Western Blots to Subclassify the Childhood Solid Tumours (en)
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| skos:prefLabel
| - Use of Brightness Wavelet Transformation for Automated Analysis of Serum Metallothioneins and Zinc-Containing-Proteins by Western Blots to Subclassify the Childhood Solid Tumours
- Use of Brightness Wavelet Transformation for Automated Analysis of Serum Metallothioneins and Zinc-Containing-Proteins by Western Blots to Subclassify the Childhood Solid Tumours (en)
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| skos:notation
| - RIV/68407700:21230/13:00207541!RIV14-MSM-21230___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/68407700:21230/13:00207541
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Signal Processing; Western-Blotting; Metallothionein; Zinc; Childhood Solid Tumours; Gel Electrophoresis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Adam, V.
- Anýž, Jiří
- Eckschlager, T.
- Kizek, R.
- Vysloužilová, Lenka
- Štěpánková, Olga
- Hraběta, J.
- Hynek, D.
- Kruseová, J.
- Křížová, S.
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
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| http://localhost/t...ganizacniJednotka
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