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  • Diamond thin films are recognize as promising material for life science and regenerative medicine due to its excellent biocompatibility and tunable surface properties by grafted molecules. Use of diamond films as antibacterial coating is less known and still evokes discussions. In this work we presents study on antibacterial properties of hydrogen and oxygen terminated diamond thin films (H- and O-diamond). As negative reference sample is used glass and copper is used as strong positive sample. Gram negative bacteria - E. Coli is used as modeling organism. The bacterial culture was grown in whole media at 37 ffiC on specimens (H-diamond, O-diamond, glass and copper) with specific area around 20 cm2. The antibacterial properties were determined by number of bacteria colonies after 24 hours incubation. Experiments on bacteria growth on copper, the well known member of antibacterial materials, confirmed its killing effect all bacteria. Oxygen grafted diamond films had a minimal impact on bacterial growth and achieved results are comparable with reference samples (glass). In comparison to the reference sample, the hydrogen grafted diamond film effectively reduced the number of bacteria by 60 %. This observation confirms that in specific case (i.e. proper surface termination on atomic leve), diamond can be classified as antibacterial material.
  • Diamond thin films are recognize as promising material for life science and regenerative medicine due to its excellent biocompatibility and tunable surface properties by grafted molecules. Use of diamond films as antibacterial coating is less known and still evokes discussions. In this work we presents study on antibacterial properties of hydrogen and oxygen terminated diamond thin films (H- and O-diamond). As negative reference sample is used glass and copper is used as strong positive sample. Gram negative bacteria - E. Coli is used as modeling organism. The bacterial culture was grown in whole media at 37 ffiC on specimens (H-diamond, O-diamond, glass and copper) with specific area around 20 cm2. The antibacterial properties were determined by number of bacteria colonies after 24 hours incubation. Experiments on bacteria growth on copper, the well known member of antibacterial materials, confirmed its killing effect all bacteria. Oxygen grafted diamond films had a minimal impact on bacterial growth and achieved results are comparable with reference samples (glass). In comparison to the reference sample, the hydrogen grafted diamond film effectively reduced the number of bacteria by 60 %. This observation confirms that in specific case (i.e. proper surface termination on atomic leve), diamond can be classified as antibacterial material. (en)
Title
  • Antibacterial studies on nanocrystalline diamond thin films
  • Antibacterial studies on nanocrystalline diamond thin films (en)
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  • Antibacterial studies on nanocrystalline diamond thin films
  • Antibacterial studies on nanocrystalline diamond thin films (en)
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  • RIV/68407700:21110/12:00196983!RIV13-MSM-21110___
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  • 122895
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  • RIV/68407700:21110/12:00196983
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  • Diamond thin film; bacteria (en)
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  • [E100384C5659]
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  • Tesárek, Pavel
  • Ryparová, Pavla
  • Kromka, A.
  • Babchenko, O.
  • Potocký, Š.
http://localhost/t...ganizacniJednotka
  • 21110
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