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  • Atomic force microscopy (AFM) in contact mode and tapping mode is employed for high resolution studies of soft organic molecules (fetal bovine serum proteins) on hard inorganic diamond substrates in solution and air. Various effects in morphology and phase measurements related to the cantilever spring constant, amplitude of tip oscillations, surface approach, tip shape and condition are demonstrated and discussed based on the proposed schematic models. We show that both diamond and proteins can be mechanically modified by Si AFM cantilever. We propose how to choose suitable cantilever type, optimize scanning parameters, recognize and minimize various artifacts, and obtain reliable AFM data both in solution and in air to reveal microscopic characteristics of protein-diamond interfaces. We also suggest that monocrystalline diamond is well defined substrate that can be applicable for fundamental studies of molecules on surfaces in general.
  • Atomic force microscopy (AFM) in contact mode and tapping mode is employed for high resolution studies of soft organic molecules (fetal bovine serum proteins) on hard inorganic diamond substrates in solution and air. Various effects in morphology and phase measurements related to the cantilever spring constant, amplitude of tip oscillations, surface approach, tip shape and condition are demonstrated and discussed based on the proposed schematic models. We show that both diamond and proteins can be mechanically modified by Si AFM cantilever. We propose how to choose suitable cantilever type, optimize scanning parameters, recognize and minimize various artifacts, and obtain reliable AFM data both in solution and in air to reveal microscopic characteristics of protein-diamond interfaces. We also suggest that monocrystalline diamond is well defined substrate that can be applicable for fundamental studies of molecules on surfaces in general. (en)
Title
  • Optimizing atomic force microscopy for characterization of diamond-protein interfaces
  • Optimizing atomic force microscopy for characterization of diamond-protein interfaces (en)
skos:prefLabel
  • Optimizing atomic force microscopy for characterization of diamond-protein interfaces
  • Optimizing atomic force microscopy for characterization of diamond-protein interfaces (en)
skos:notation
  • RIV/68378271:_____/11:00375015!RIV12-AV0-68378271
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GAP108/11/0794), P(KAN400100701), P(LC06040), P(LC510), Z(AV0Z10100521)
http://linked.open...iv/cisloPeriodika
  • Apr.
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 218795
http://linked.open...ai/riv/idVysledku
  • RIV/68378271:_____/11:00375015
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • atomic force microscopy (AFM); nanocrystalline diamond; oxygen-terminated diamond; hydrogen-terminated diamond; proteins; fetal bovine serum (FBS) (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [BBE43BBA4337]
http://linked.open...i/riv/nazevZdroje
  • Nanoscale Research Letters
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 6
http://linked.open...iv/tvurceVysledku
  • Kromka, Alexander
  • Rezek, Bohuslav
  • Ukraintsev, Egor
http://linked.open...ain/vavai/riv/wos
  • 000292289900005
http://linked.open...n/vavai/riv/zamer
issn
  • 1931-7573
number of pages
http://bibframe.org/vocab/doi
  • 10.1186/1556-276X-6-337
is http://linked.open...avai/riv/vysledek of
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