About: Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin

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About: Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Microtubules, polymers of the heterodimeric protein αβ-tubulin, are indispensable for many cellular activities such as maintenance of cell shape, division, migration, and ordered vesicle transport. In vitro assays to study microtubule functions and their regulation by associated proteins require the availability of assembly-competent purified tubulin. However, tubulin is a thermolabile protein that rapidly converts into non-polymerizing state. For this reason it is usually stored at 80 °C to preserve its conformation and polymerization properties. In this chapter we describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling evaluation of tubulin functions in rehydrated samples. Microtubules, polymers of the heterodimeric protein αβ-tubulin, are indispensable for many cellular activities such as maintenance of cell shape, division, migration, and ordered vesicle transport. In vitro assays to study microtubule functions and their regulation by associated proteins require the availability of assembly-competent purified tubulin. However, tubulin is a thermolabile protein that rapidly converts into non-polymerizing state. For this reason it is usually stored at 80 °C to preserve its conformation and polymerization properties. In this chapter we describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling evaluation of tubulin functions in rehydrated samples. (en)
Title	Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin (en)
skos:prefLabel	Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin (en)
skos:notation	RIV/68378050:_____/14:00434353!RIV15-GA0-68378050
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP302/10/1701), P(GPP302/11/P709), P(LH12050)
http://linked.open...iv/cisloPeriodika	February
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Dráber, Pavel Dráberová, Eduarda Sulimenko, Tetyana Sulimenko, Vadym
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	46988
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/14:00434353
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Freeze-drying; Microtubules; Stability; Trehalose; Tubulin (en)
http://linked.open.../riv/klicoveSlovo	Trehalose Microtubules Stability Freeze-drying Tubulin
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[4724B36253F9]
http://linked.open...i/riv/nazevZdroje	Methods in Molecular Biology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Analysis of microtubular changes during activation of mast cells Regulation of microtubule formation in brain cancer cells Analysis of functional differences between gamma-tubulins
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	1129
http://linked.open...iv/tvurceVysledku	Dráber, Pavel Dráberová, Eduarda Sulimenko, Tetyana Sulimenko, Vadym
http://linked.open...ain/vavai/riv/wos	000337104300033
issn	1064-3745
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-977-2_32

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