About: Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin

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About: Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The chapter 32 covers an elegant method for lyphilization of tubulin. Authors describe step by step the protocol for preparation of freeze-drying (lyophilizated) sample of tubulin. Tubulin is heterodimeric protein that composes microtubules as part of cytoskeleton of the cell. However, tubulin is a thermolabile protein that rapidly converts into non-polymerazing state. For this reason it is usually stored at -80°C to preserve its conformation and polymerization properties. In this chapter authors describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling the evaluation of tubulin functions in rehydrated samples. The chapter also includes images documenting the effect of trehalose on the functionality of the rehydrated tubulin suitable for in vitro studies. The chapter 32 covers an elegant method for lyphilization of tubulin. Authors describe step by step the protocol for preparation of freeze-drying (lyophilizated) sample of tubulin. Tubulin is heterodimeric protein that composes microtubules as part of cytoskeleton of the cell. However, tubulin is a thermolabile protein that rapidly converts into non-polymerazing state. For this reason it is usually stored at -80°C to preserve its conformation and polymerization properties. In this chapter authors describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling the evaluation of tubulin functions in rehydrated samples. The chapter also includes images documenting the effect of trehalose on the functionality of the rehydrated tubulin suitable for in vitro studies. (en)
Title	Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin (en)
skos:prefLabel	Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin (en)
skos:notation	RIV/68378050:_____/14:00431748!RIV15-GA0-68378050
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP302/10/1701), P(GAP302/12/1673), P(GPP302/11/P709), P(LH12050)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Dráber, Pavel Dráberová, Eduarda Sulimenko, Tetyana Sulimenko, Vadym
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	46989
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/14:00431748
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Freeze-drying; Microtubules; Stability; Trehalose; Tubulin (en)
http://linked.open.../riv/klicoveSlovo	Trehalose Microtubules Stability Freeze-drying Tubulin
http://linked.open...ontrolniKodProRIV	[699FB0FAF0FB]
http://linked.open...i/riv/mistoVydani	New York
http://linked.open...i/riv/nazevZdroje	Protein Downstream Processing
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...v/pocetStranKnihy	555 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Role of gamma-tubulin complexes in regulation of cell cycle progression Analysis of microtubular changes during activation of mast cells Regulation of microtubule formation in brain cancer cells Analysis of functional differences between gamma-tubulins
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Dráber, Pavel Dráberová, Eduarda Sulimenko, Tetyana Sulimenko, Vadym
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-977-2_32
http://purl.org/ne...btex#hasPublisher	Humana Press INC
https://schema.org/isbn	978-1-62703-976-5

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