About: The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing

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About: The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Brd2 is a member of the bromodomain extra terminal (BET) protein family, which consists of four chromatin-interacting proteins that regulate gene expression. Each BET protein contains two N-terminal bromodomains, which recognize acetylated histones, and the C-terminal protein-protein interaction domain. Using a genome-wide screen, we identify 1450 genes whose transcription is regulated by Brd2. In addition, almost 290 genes change their alternative splicing pattern upon Brd2 depletion. Brd2 is specifically localized at promoters of target genes, and our data show that Brd2 interaction with chromatin cannot be explained solely by histone acetylation. Using coimmunoprecipitation and live-cell imaging, we show that the C-terminal part is crucial for Brd2 association with chromatin. Live-cell microscopy also allows us to map the average binding time of Brd2 to chromatin and quantify the contributions of individual Brd2 domains to the interaction with chromatin. Finally, we show that bromodomains and the C-terminal domain are equally important for transcription and splicing regulation, which correlates with the role of these domains in Brd2 binding to chromatin. Brd2 is a member of the bromodomain extra terminal (BET) protein family, which consists of four chromatin-interacting proteins that regulate gene expression. Each BET protein contains two N-terminal bromodomains, which recognize acetylated histones, and the C-terminal protein-protein interaction domain. Using a genome-wide screen, we identify 1450 genes whose transcription is regulated by Brd2. In addition, almost 290 genes change their alternative splicing pattern upon Brd2 depletion. Brd2 is specifically localized at promoters of target genes, and our data show that Brd2 interaction with chromatin cannot be explained solely by histone acetylation. Using coimmunoprecipitation and live-cell imaging, we show that the C-terminal part is crucial for Brd2 association with chromatin. Live-cell microscopy also allows us to map the average binding time of Brd2 to chromatin and quantify the contributions of individual Brd2 domains to the interaction with chromatin. Finally, we show that bromodomains and the C-terminal domain are equally important for transcription and splicing regulation, which correlates with the role of these domains in Brd2 binding to chromatin. (en)
Title	The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing (en)
skos:prefLabel	The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing (en)
skos:notation	RIV/68378050:_____/13:00422977!RIV14-GA0-68378050
http://linked.open...avai/predkladatel	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP305/10/0424), P(GBP208/12/G016), P(GBP305/12/G034), P(KAN200520801)
http://linked.open...iv/cisloPeriodika	22
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Staněk, David Hnilicová, Jarmila Dušková, Eva Stejskalová, Eva Hozeifi, Samira
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	67447
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/13:00422977
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Brd2; alternative splicing; chromatin (en)
http://linked.open.../riv/klicoveSlovo	Brd2 chromatin alternative splicing
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[B8C134D51C65]
http://linked.open...i/riv/nazevZdroje	Molecular Biology of the Cell
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Controlling structure and function of biomolecules at the molecular scale: theory meets experiment Centre for RNA Biology Targeted expression and transport of bioactive molecules Regulation of alternative splicing via chromatin acetylation
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	24
http://linked.open...iv/tvurceVysledku	Hof, Martin Stejskalová, Eva Humpolíčková, Jana Dušková, Eva Hnilicová, Jarmila Hozeifi, Samira Staněk, David Poser, I.
http://linked.open...ain/vavai/riv/wos	000328124600007
issn	1059-1524
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1091/mbc.E13-06-0303

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