About: A lens-specific co-injection marker for medaka transgenesis

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Recent advances in fish transgenesis allow generation of transgenic animals at high numbers and efficiency of germ-line transmission. To facilitate the procedure, a suitable co-injection marker may be very helpful for preselection of transgenic embryos. For this purpose, a lens-specific marker appears to be a suitable candidate since the lens is a well defined tissue positioned at the periphery and is easily accessible for examination of reporter gene expression. Here, we constructed reporter vectors based on mouse gamma-F crystallin (mF-Cry) promoter driving heterologous expression of the reporter gene at a high level and very specifically to the lens allowing an easy sorting of transgenics. Recent advances in fish transgenesis allow generation of transgenic animals at high numbers and efficiency of germ-line transmission. To facilitate the procedure, a suitable co-injection marker may be very helpful for preselection of transgenic embryos. For this purpose, a lens-specific marker appears to be a suitable candidate since the lens is a well defined tissue positioned at the periphery and is easily accessible for examination of reporter gene expression. Here, we constructed reporter vectors based on mouse gamma-F crystallin (mF-Cry) promoter driving heterologous expression of the reporter gene at a high level and very specifically to the lens allowing an easy sorting of transgenics. (en)
Title	A lens-specific co-injection marker for medaka transgenesis A lens-specific co-injection marker for medaka transgenesis (en)
skos:prefLabel	A lens-specific co-injection marker for medaka transgenesis A lens-specific co-injection marker for medaka transgenesis (en)
skos:notation	RIV/68378050:_____/10:00346841!RIV11-MSM-68378050
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(1M0520), P(IAA500520604), P(IAA500520908), S, Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Pavlů, Barbora Smolíková, Jana Kozmik, Zbyněk Vopálenský, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	244662
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/10:00346841
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	lens; transgenic; crystallin (en)
http://linked.open.../riv/klicoveSlovo	lens transgenic crystallin
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[0F9845D75185]
http://linked.open...i/riv/nazevZdroje	Biotechniques
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Applied Genomics Developmental Genetics of Amphioxus:Insight into Evolutionary Origin of Vertebrates The role of Pax genes in eye evolution
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Kozmik, Zbyněk Vopálenský, Pavel Růžičková, Jana Pavlů, Barbora
http://linked.open...ain/vavai/riv/wos	000277562300011
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0736-6205
number of pages	2 (xsd:int)
is http://linked.open...avai/riv/vysledek of	A lens-specific co-injection marker for medaka transgenesis A lens-specific co-injection marker for medaka transgenesis A lens-specific co-injection marker for medaka transgenesis

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