About: Assessment of in vitro drug resistance of human breast cancer cells subcultured from biopsy specimens.

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About: Assessment of in vitro drug resistance of human breast cancer cells subcultured from biopsy specimens. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The low cellular yield of a breast cancer sample is a limiting factor for in vitro chemoresistance testing. The use of in vitro serially cultured cells can help overcome this obstacle. In vitro drug resistance of cells cultured from mammary carcinomas by the 3T3 feeder-layer technique was tested by the MTT assay. Out of the 33 tested cultures, 9 were derived from cells obtained from true-cut biopsies of primary tumours, with sample volume less than 0.03 cm3. The cultures were treated with 6 anticancer drugs. The chemoresistance of cultured cells was monitored by the surviving cell fraction. Paclitaxel and cisplatin were the most potent drugs. Gemcitabine, vinorelbine and mafosfamide were the least potent drugs. Doxorubicin and gemcitabine most frequently failed to completely metabolically inhibit 100% of cultured cells. Combination of the optimised feeder-layer cultivation technique and the MTT test permits extensive drug resistance testing from very small breast cancer samples. The low cellular yield of a breast cancer sample is a limiting factor for in vitro chemoresistance testing. The use of in vitro serially cultured cells can help overcome this obstacle. In vitro drug resistance of cells cultured from mammary carcinomas by the 3T3 feeder-layer technique was tested by the MTT assay. Out of the 33 tested cultures, 9 were derived from cells obtained from true-cut biopsies of primary tumours, with sample volume less than 0.03 cm3. The cultures were treated with 6 anticancer drugs. The chemoresistance of cultured cells was monitored by the surviving cell fraction. Paclitaxel and cisplatin were the most potent drugs. Gemcitabine, vinorelbine and mafosfamide were the least potent drugs. Doxorubicin and gemcitabine most frequently failed to completely metabolically inhibit 100% of cultured cells. Combination of the optimised feeder-layer cultivation technique and the MTT test permits extensive drug resistance testing from very small breast cancer samples. (en)
Title	Assessment of in vitro drug resistance of human breast cancer cells subcultured from biopsy specimens. Assessment of in vitro drug resistance of human breast cancer cells subcultured from biopsy specimens. (en)
skos:prefLabel	Assessment of in vitro drug resistance of human breast cancer cells subcultured from biopsy specimens. Assessment of in vitro drug resistance of human breast cancer cells subcultured from biopsy specimens. (en)
skos:notation	RIV/68378050:_____/03:23033093!RIV/2004/AV0/A23004/N
http://linked.open.../vavai/riv/strany	2593;2600
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(NC6734), Z(AV0Z5052915)
http://linked.open...iv/cisloPeriodika	3B
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Veselý, Pavel Chaloupková, Alena Krásná, Luboslava Matoušková, Eva Taišlová, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	599175
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/03:23033093
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Breast cancer; 3T3 feeder-layer culture technique; MTT test (en)
http://linked.open.../riv/klicoveSlovo	Breast cancer MTT test 3T3 feeder-layer culture technique
http://linked.open...odStatuVydavatele	GR - Řecká republika
http://linked.open...ontrolniKodProRIV	[F44122E8BA62]
http://linked.open...i/riv/nazevZdroje	Anticancer Research
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Testing the individualisation possibility of the treatment of ovarian tumours by using prediction of chemoresistance and biosensitivity in vitro
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	23
http://linked.open...iv/tvurceVysledku	Veselý, Pavel Matoušková, Eva Petruželka, L. Chaloupková, Alena Pavlíková, Luboslava Daneš, J. Netíková, I. Taišlová, Eva Zimovjanová, M.
http://linked.open...n/vavai/riv/zamer	Regulace a signální cesty uplatňující se v genové expresi, imunitě, onkogenezi, replikaci virů, tvorbě buněčných struktur, chování buněk, vývoji a oplození
issn	0250-7005
number of pages	8 (xsd:int)

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