About: Urinary 8-oxo-7,8-dihydroguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	ELISA is widely used for urinary 8-oxo-7.8-dihydro-2´-deoxyguanosine (8-oxodG) analysis. However, a lack of agreement between ELISA and chromatographic methods confines its application to the assessment of relative urinary 8-oxodG level. We investigated various ELISA modifications, seeking optimal conditions that would yield a good agreement between ELISA and HPLC-MS/MS. It was found, that purification of urine by solid phase extraction, then incubation with the anti-8-oxodG antibody at 40 C overnight and subsequent normalization of 8-oxodG levels per urinary creatinine resulted in a near-perfect correlation and agreement in mean levels between ELISA and HPLC-MS/MS. Our data show that, after introduction of a simple modification, ELISA quantification urinary 8-oxodG substantially improves. This method represents a significant advance for the ELISA, and encouraging its use in more studies adding to our knowledge of the role of this biomarker of oxidative stress in health and disease. ELISA is widely used for urinary 8-oxo-7.8-dihydro-2´-deoxyguanosine (8-oxodG) analysis. However, a lack of agreement between ELISA and chromatographic methods confines its application to the assessment of relative urinary 8-oxodG level. We investigated various ELISA modifications, seeking optimal conditions that would yield a good agreement between ELISA and HPLC-MS/MS. It was found, that purification of urine by solid phase extraction, then incubation with the anti-8-oxodG antibody at 40 C overnight and subsequent normalization of 8-oxodG levels per urinary creatinine resulted in a near-perfect correlation and agreement in mean levels between ELISA and HPLC-MS/MS. Our data show that, after introduction of a simple modification, ELISA quantification urinary 8-oxodG substantially improves. This method represents a significant advance for the ELISA, and encouraging its use in more studies adding to our knowledge of the role of this biomarker of oxidative stress in health and disease. (en)
Title	Urinary 8-oxo-7,8-dihydroguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS Urinary 8-oxo-7,8-dihydroguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS (en)
skos:prefLabel	Urinary 8-oxo-7,8-dihydroguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS Urinary 8-oxo-7,8-dihydroguanosine values determined by a modified ELISA improves agreement with HPLC-MS/MS (en)
skos:notation	RIV/68378041:_____/13:00398237!RIV14-GA0-68378041
http://linked.open...avai/predkladatel	Ústav experimentální medicíny Akademie věd České republiky, v. v. i.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA13-13458S), P(GAP503/11/0084)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Šrám, Radim Rössner ml., Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální medicíny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	112877
http://linked.open...ai/riv/idVysledku	RIV/68378041:_____/13:00398237
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	8-oxodG; urine; ELISA (en)
http://linked.open.../riv/klicoveSlovo	8-oxodG ELISA urine
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[2BD587AE05D8]
http://linked.open...i/riv/nazevZdroje	Biochemical and Biophysical Research Communications
http://linked.open...in/vavai/riv/obor	DN
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Impact of air pollution to genome of newborns The effect of polycyclic aromatic hydrocarbons and particulate matter on DNA damage and DNA repair mechanisms in vitro
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	440
http://linked.open...iv/tvurceVysledku	Rössner ml., Pavel Šrám, Radim Mistry, V. Cooke, M. S. Singh, R.
http://linked.open...ain/vavai/riv/wos	000326905800045
issn	0006-291X
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.bbrc.2013.09.133

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