About: Searching for active ribosomal genes in situ:light microscopy in light of the electron beam.

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About: Searching for active ribosomal genes in situ:light microscopy in light of the electron beam. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	To improve the LM resolution, computational methods based on removal of the image blur are frequently implemented in cell biology studies. One of the standard deblurring approaches is image restoration throught deconvolution algorithms. We demonstrate that, in the specific case of the fluorescence mapping of active ribosomal genes in HeLa cell nucleoli, restoration generates a biased result. On restoration of model images, we demostrate the difficulties of one of the most effective deconvolution algorithms during the restoration of ring-shaped fluorescent objects of a diameter comparable to the microscope resolution limit. To improve the LM resolution, computational methods based on removal of the image blur are frequently implemented in cell biology studies. One of the standard deblurring approaches is image restoration throught deconvolution algorithms. We demonstrate that, in the specific case of the fluorescence mapping of active ribosomal genes in HeLa cell nucleoli, restoration generates a biased result. On restoration of model images, we demostrate the difficulties of one of the most effective deconvolution algorithms during the restoration of ring-shaped fluorescent objects of a diameter comparable to the microscope resolution limit. (en)
Title	Searching for active ribosomal genes in situ:light microscopy in light of the electron beam. Searching for active ribosomal genes in situ:light microscopy in light of the electron beam. (en)
skos:prefLabel	Searching for active ribosomal genes in situ:light microscopy in light of the electron beam. Searching for active ribosomal genes in situ:light microscopy in light of the electron beam. (en)
skos:notation	RIV/68378041:_____/02:19023084!RIV/2003/GA0/A19003/N
http://linked.open.../vavai/riv/strany	227;231
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA304/00/1481), P(GA304/00/D035), P(GA304/01/0729), P(IAA5039103), Z(AV0Z5039906), Z(MSM 111100003)
http://linked.open...iv/cisloPeriodika	N/A
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Raška, Ivan Malinský, Jan Koberna, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální medicíny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	663097
http://linked.open...ai/riv/idVysledku	RIV/68378041:_____/02:19023084
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence; image restoration; ribosomal genes (en)
http://linked.open.../riv/klicoveSlovo	image restoration fluorescence ribosomal genes
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[11D31E5800AF]
http://linked.open...i/riv/nazevZdroje	Journal of Structural Biology
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamic structure and function in the cell nucleus Deconvolution image restoration in the studies of the cell nucleus architecture Large scale organization of chromatin Dynamics of Spliceosome assembly
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	140
http://linked.open...iv/tvurceVysledku	Raška, Ivan Malínský, Jan Koberna, Karel Štulík, J. Bednár, J.
http://linked.open...n/vavai/riv/zamer	The molecular and cellular basis of selected human diseases, mechanisms of normal and pathological function, principles of diagnosis and therapy http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20111100003
issn	1047-8477
number of pages	5 (xsd:int)

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