About: Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	During the years 1999-2002, a total of 4,898 individuals of 26 species of hematophagous insects (4,149 mosquitoes, 583 black flies, and 166 tabanid flies) was examined for the presence of spirochetes using dark-field microscopy. There was an overall recovery of spirochetes from the midguts of Culicidae and Simuliidae of 23.5% and 11.4%, respectively. Spirochetes were not detected in Tabanidae. Seven spirochetal strains have been successfully recovered from mosquitoes and black flies: BR149 (Culex pipiens), BR151 (Cx. pipiens), BR173 (Cx. pipiens), BR177 (Cx. pipiens), BR193 (Aedes cinereus), BR208 (Cx. pipiens), and BR231 (Simulium noelleri). The strains have been adapted to laboratory conditions (BSK-H Complete medium). Their preliminary determination based on 16S rRNA gene sequencing has shown that they differ from the Lyme disease spirochete Borrelia burgdorferi sensu lato as well as other members of the Order Spirochaetales indicating novel bacterial species in the Family Spirochaetaceae. During the years 1999-2002, a total of 4,898 individuals of 26 species of hematophagous insects (4,149 mosquitoes, 583 black flies, and 166 tabanid flies) was examined for the presence of spirochetes using dark-field microscopy. There was an overall recovery of spirochetes from the midguts of Culicidae and Simuliidae of 23.5% and 11.4%, respectively. Spirochetes were not detected in Tabanidae. Seven spirochetal strains have been successfully recovered from mosquitoes and black flies: BR149 (Culex pipiens), BR151 (Cx. pipiens), BR173 (Cx. pipiens), BR177 (Cx. pipiens), BR193 (Aedes cinereus), BR208 (Cx. pipiens), and BR231 (Simulium noelleri). The strains have been adapted to laboratory conditions (BSK-H Complete medium). Their preliminary determination based on 16S rRNA gene sequencing has shown that they differ from the Lyme disease spirochete Borrelia burgdorferi sensu lato as well as other members of the Order Spirochaetales indicating novel bacterial species in the Family Spirochaetaceae. (en)
Title	Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic (en)
skos:prefLabel	Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic (en)
skos:notation	RIV/68081766:_____/10:00345538!RIV11-GA0-68081766
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA206/00/1234), P(GA206/03/0726), Z(AV0Z60930519)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Mendel, Jan Halouzka, Jiří Šikutová, Silvie Rudolf, Ivo
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav biologie obratlovců AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	275455
http://linked.open...ai/riv/idVysledku	RIV/68081766:_____/10:00345538
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Spirochetes; mosquitoes; black flies; tabanid flies; Czech Republic (en)
http://linked.open.../riv/klicoveSlovo	Spirochetes Czech Republic mosquitoes black flies tabanid flies
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[90A9A43E9300]
http://linked.open...i/riv/nazevZdroje	Journal of Vector Ecology
http://linked.open...in/vavai/riv/obor	FN
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	The study of selected groups of haematophagous diptera as possible carriers of spirochaetes including pathogenic species Ecology of emerging arthropod-borne microorganisms
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	35
http://linked.open...iv/tvurceVysledku	Mendel, Jan Rudolf, Ivo Halouzka, Jiří Šikutová, Silvie Knoz, J.
http://linked.open...ain/vavai/riv/wos	000278574900007
http://linked.open...n/vavai/riv/zamer	Biodiverzita a ekologie obratlovců: Strategie ochrany a využívání přírodních populací
issn	1081-1710
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic

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