About: The Low-Temperature Method for Study of Coniferous Tissues in the Environmental Scanning Electron Microscope

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About: The Low-Temperature Method for Study of Coniferous Tissues in the Environmental Scanning Electron Microscope Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The use of non-standard low-temperature conditions in environmental scanning electron microscopy might be promising for the observation of coniferous tissues in their native state. This study is aimed to analyse and evaluate the method based on the principle of lowtemperature sample stabilization. We demonstrate that the upper mucous layer is sublimed and a microstructure of the sample surface can be observed with higher resolution at lower gas pressure conditions, thanks to a low-temperature method. An influence of the low-temperature method on sample stability was also studied. The results indicate that high-moisture conditions are not suitable for this method and often cause the collapse of samples. The potential improvement of stability to beam damage has been demonstrated by long-time observation at different operation parameters. We finally show high applicability of the low-temperature method on different types of conifers and Oxalis acetosella. The use of non-standard low-temperature conditions in environmental scanning electron microscopy might be promising for the observation of coniferous tissues in their native state. This study is aimed to analyse and evaluate the method based on the principle of lowtemperature sample stabilization. We demonstrate that the upper mucous layer is sublimed and a microstructure of the sample surface can be observed with higher resolution at lower gas pressure conditions, thanks to a low-temperature method. An influence of the low-temperature method on sample stability was also studied. The results indicate that high-moisture conditions are not suitable for this method and often cause the collapse of samples. The potential improvement of stability to beam damage has been demonstrated by long-time observation at different operation parameters. We finally show high applicability of the low-temperature method on different types of conifers and Oxalis acetosella. (en)
Title	The Low-Temperature Method for Study of Coniferous Tissues in the Environmental Scanning Electron Microscope The Low-Temperature Method for Study of Coniferous Tissues in the Environmental Scanning Electron Microscope (en)
skos:prefLabel	The Low-Temperature Method for Study of Coniferous Tissues in the Environmental Scanning Electron Microscope The Low-Temperature Method for Study of Coniferous Tissues in the Environmental Scanning Electron Microscope (en)
skos:notation	RIV/68081731:_____/15:00439221!RIV15-GA0-68081731
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA14-22777S)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Flodrová, Eva Hřib, Jiří Neděla, Vilém
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav přístrojové techniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	252
http://linked.open...ai/riv/idVysledku	RIV/68081731:_____/15:00439221
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	methodology; conifer; embryogenic tissue; extracellular matrix; Oxalis acetosella (en)
http://linked.open.../riv/klicoveSlovo	conifer methodology Oxalis acetosella embryogenic tissue extracellular matrix
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[832F605EDCE1]
http://linked.open...i/riv/nazevZdroje	Microscopy Research Technique
http://linked.open...in/vavai/riv/obor	JA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Studium interakcí elektronů s plynem v podmínkách tlakového spádu nízkoenergiového environmentálního rastrovacího elektronového mikroskopu.
http://linked.open...UplatneniVysledku	2015
http://linked.open...v/svazekPeriodika	78
http://linked.open...iv/tvurceVysledku	Neděla, Vilém Tihlaříková, Eva Hřib, Jiří
http://linked.open...ain/vavai/riv/wos	000346767800004
issn	1059-910X
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jemt.22439

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