About: Monitoring the influence of antibiotic exposure using Raman spectroscopy

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Here we report on combination of the data obtained from MICs (minimum inhibitory concentrations) with infor- mation of microoragnisms fingerprint provided by Raman spectroscopy. In our feasibility study we could follow mechanisms of the bacteriostatic versus bactericidal action on biofilm-positive Staphylococcus epidermidis simply by monitoring Raman bands corresponding to DNA translating the changes introduced by selected antibiotics. The Raman spectra of Staphylococcus epidermidis treated with a bacteriostatic agent show little effect on DNA which is in contrast with the action of a bactericidal agent where decreased in dedicated Raman spectra signal strength suggests DNA fragmentation. Moreover, we demonstrate that Raman tweezers are indeed able to distinguish strains of biofilm-forming (biofilm-positive) and biofilm-negative Staphylococcus epidermidis strains using principal component analysis (PCA). Here we report on combination of the data obtained from MICs (minimum inhibitory concentrations) with infor- mation of microoragnisms fingerprint provided by Raman spectroscopy. In our feasibility study we could follow mechanisms of the bacteriostatic versus bactericidal action on biofilm-positive Staphylococcus epidermidis simply by monitoring Raman bands corresponding to DNA translating the changes introduced by selected antibiotics. The Raman spectra of Staphylococcus epidermidis treated with a bacteriostatic agent show little effect on DNA which is in contrast with the action of a bactericidal agent where decreased in dedicated Raman spectra signal strength suggests DNA fragmentation. Moreover, we demonstrate that Raman tweezers are indeed able to distinguish strains of biofilm-forming (biofilm-positive) and biofilm-negative Staphylococcus epidermidis strains using principal component analysis (PCA). (en)
Title	Monitoring the influence of antibiotic exposure using Raman spectroscopy Monitoring the influence of antibiotic exposure using Raman spectroscopy (en)
skos:prefLabel	Monitoring the influence of antibiotic exposure using Raman spectroscopy Monitoring the influence of antibiotic exposure using Raman spectroscopy (en)
skos:notation	RIV/68081731:_____/14:00434960!RIV15-GA0-68081731
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED0017/01/01), P(GAP205/11/1687), P(LO1212), P(NT13242)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Ježek, Jan Zemánek, Pavel Samek, Ota Šerý, Mojmír Jákl, Petr Bernatová, Silvie Šiler, Martin
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav přístrojové techniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	30432
http://linked.open...ai/riv/idVysledku	RIV/68081731:_____/14:00434960
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Raman spectroscopy; Fingerprint recognition; Principal component analysis (en)
http://linked.open.../riv/klicoveSlovo	Fingerprint recognition Raman spectroscopy Principal component analysis
http://linked.open...ontrolniKodProRIV	[059B09E4F01A]
http://linked.open...v/mistoKonaniAkce	San Francisco
http://linked.open...i/riv/mistoVydani	Bellingham
http://linked.open...i/riv/nazevZdroje	Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry (Proceedings of SPIE 8939)
http://linked.open...in/vavai/riv/obor	BH
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	ALISI - Centre of advanced diagnostic methods and technologies APPLICATION LABORATORIES OF ADVANCED MICROTECHNOLOGIES AND NANOTECHNOLOGIES Poly-microbial biofilm - the cause of chronic urinary infections and therapy failure: cultivation and PCR-DGGE diagnostics, importance and possibilities of affection Non-invasive contactless methods of identification and characterization of living microorganisms by optical spectroscopy and micromanipulation
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Ježek, Jan Samek, Ota Zemánek, Pavel Bernatová, Silvie Jákl, Petr Šerý, Mojmír Šiler, Martin Růžička, F.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000334098200015
http://linked.open.../riv/zahajeniAkce	2014-02-01 (xsd:date)
issn	0277-786X
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1117/12.2038595
http://purl.org/ne...btex#hasPublisher	SPIE
https://schema.org/isbn	9780819498526

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