About: Detecting sequential bond formation using three-dimensional thermal fluctuation analysis

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We present a novel experimental method that solves two key problems in nondestructive mechanical studies of small biomolecules at the single-molecule level, namely the confirmation of single-molecule conditions and the discrimination against nonspecific binding. A biotin avidin ligand-receptor couple is spanned between a glass slide and a 1 mu m latex particles using short linker molecules. Optical tweezers are used to initiate bond formation and to follow the particles thermal position fluctuations with nanometer spatial and microsecond temporal resolution. Here we show that each step in the specific binding process leads to an abrupt change in the magnitude of the particle's thermal position fluctuations, allowing us to count the number of bonds formed one by one. Moreover, three-dimensional position histograms calculated from the particle's fluctuations can be separated into well-defined categories reflecting different binding conditions. We present a novel experimental method that solves two key problems in nondestructive mechanical studies of small biomolecules at the single-molecule level, namely the confirmation of single-molecule conditions and the discrimination against nonspecific binding. A biotin avidin ligand-receptor couple is spanned between a glass slide and a 1 mu m latex particles using short linker molecules. Optical tweezers are used to initiate bond formation and to follow the particles thermal position fluctuations with nanometer spatial and microsecond temporal resolution. Here we show that each step in the specific binding process leads to an abrupt change in the magnitude of the particle's thermal position fluctuations, allowing us to count the number of bonds formed one by one. Moreover, three-dimensional position histograms calculated from the particle's fluctuations can be separated into well-defined categories reflecting different binding conditions. (en)
Title	Detecting sequential bond formation using three-dimensional thermal fluctuation analysis Detecting sequential bond formation using three-dimensional thermal fluctuation analysis (en)
skos:prefLabel	Detecting sequential bond formation using three-dimensional thermal fluctuation analysis Detecting sequential bond formation using three-dimensional thermal fluctuation analysis (en)
skos:notation	RIV/68081731:_____/09:00333759!RIV10-AV0-68081731
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z20650511)
http://linked.open...iv/cisloPeriodika	9-10
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Jonáš, Alexandr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav přístrojové techniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	309762
http://linked.open...ai/riv/idVysledku	RIV/68081731:_____/09:00333759
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	biophysics; mechanical properties; optical tweezers; single-molecule studies; thermal fluctuations (en)
http://linked.open.../riv/klicoveSlovo	single-molecule studies thermal fluctuations mechanical properties optical tweezers biophysics
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[4847555E2947]
http://linked.open...i/riv/nazevZdroje	ChemPhysChem
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	10
http://linked.open...iv/tvurceVysledku	Jonáš, Alexandr Huang, R. Bartsch, T. Fisinger, S. Florin, E. L. Kochanczyk, M. D.
http://linked.open...ain/vavai/riv/wos	000267928100028
http://linked.open...n/vavai/riv/zamer	Research into experimental methods for examination of the physical properties of matter and their application in advanced technologies
issn	1439-4235
number of pages	7 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Detecting sequential bond formation using three-dimensional thermal fluctuation analysis Detecting sequential bond formation using three-dimensional thermal fluctuation analysis Detecting sequential bond formation using three-dimensional thermal fluctuation analysis Detecting sequential bond formation using three-dimensional thermal fluctuation analysis

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