About: Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study

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Description	N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the structure of both oligosaccharide and peptide moiety. However, this method has so far been less commonly used. Since glycopeptides hardly ionize during MS analysis in the presence of non-glycosylated peptides, they need to be separated from the complex peptide mixture. In this study, the glycopeptides enrichment, purification and analysis methods were successfully optimized on two standard N-glycoproteins. Concanavalin A (ConA) lectin tips were used for glycopeptide capturing, and obtained fractions were purified on carbon tips and analyzed using MALDI-TOF mass spectrometry. The differences in the CID fragmentation of certain types of glycopeptides were found. This technique was then applied to glycopeptide analysis of barley grain and malt proteins. Several barley glycopeptides were found, however, their identification was very difficult. More proteins separation techniques will be required before this enrichment procedure in further studies. N-glycosylation is the most frequently studied plant protein post-translational modification. The analysis of Nglycopeptides after protein proteolytic digestion offers information about the structure of both oligosaccharide and peptide moiety. However, this method has so far been less commonly used. Since glycopeptides hardly ionize during MS analysis in the presence of non-glycosylated peptides, they need to be separated from the complex peptide mixture. In this study, the glycopeptides enrichment, purification and analysis methods were successfully optimized on two standard N-glycoproteins. Concanavalin A (ConA) lectin tips were used for glycopeptide capturing, and obtained fractions were purified on carbon tips and analyzed using MALDI-TOF mass spectrometry. The differences in the CID fragmentation of certain types of glycopeptides were found. This technique was then applied to glycopeptide analysis of barley grain and malt proteins. Several barley glycopeptides were found, however, their identification was very difficult. More proteins separation techniques will be required before this enrichment procedure in further studies. (en)
Title	Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study (en)
skos:prefLabel	Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study Optimization of N-glycopeptides analysis methods and their preliminary application to barley proteins study (en)
skos:notation	RIV/68081715:_____/13:00422562!RIV14-MSM-68081715
http://linked.open...avai/predkladatel	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(EE2.3.20.0182), P(GPP503/12/P395)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Flodrová, Dana Benkovská, Dagmar Bobálová, Janette Laštovičková, Markéta
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	94481
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/13:00422562
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	glycosylation; post-translational modification; barley; N-glycopetptides (en)
http://linked.open.../riv/klicoveSlovo	N-glycopetptides post-translational modification barley glycosylation
http://linked.open...ontrolniKodProRIV	[1D2E10FFD311]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Chemické Listy. Roč. 107, Issue s3.
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of Multidisciplinary Research and Educational Centrum for Bioanalytical Technologies Searching for glycosylated allergens in plants important for food chemistry via modern proteomic approaches
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Benkovská, Dagmar Bobálová, Janette Flodrová, Dana Laštovičková, Markéta
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open...ain/vavai/riv/wos	000328730800005
http://linked.open.../riv/zahajeniAkce	2013-11-12 (xsd:date)
issn	1213-7103
number of pages	3 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Česká společnost chemická

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