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  • There are several crucial steps that have to be followed to ensure successful secreted proteome detection and identification. Simultaneously with the optimization of the experimental conditions for various cell type culturing and subsequent cell conditioning to obtain conditioned medium with secreted proteins in vitro, the analytical separation methods for fractionation of complex protein mixture and mass spectrometry for protein identification are of high importance. Although optimized for neonatal cardiac myocytes, the general steps described in the following chapter can be adapted to other cell types as well.
  • There are several crucial steps that have to be followed to ensure successful secreted proteome detection and identification. Simultaneously with the optimization of the experimental conditions for various cell type culturing and subsequent cell conditioning to obtain conditioned medium with secreted proteins in vitro, the analytical separation methods for fractionation of complex protein mixture and mass spectrometry for protein identification are of high importance. Although optimized for neonatal cardiac myocytes, the general steps described in the following chapter can be adapted to other cell types as well. (en)
Title
  • Optimized method for identification of the proteomes secreted by cardiac cells
  • Optimized method for identification of the proteomes secreted by cardiac cells (en)
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  • Optimized method for identification of the proteomes secreted by cardiac cells
  • Optimized method for identification of the proteomes secreted by cardiac cells (en)
skos:notation
  • RIV/68081715:_____/13:00392231!RIV14-AV0-68081715
http://linked.open...avai/predkladatel
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  • I
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  • 1005
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  • 94514
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  • RIV/68081715:_____/13:00392231
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  • cardiac cells; secreted proteins; proteomic technology (en)
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  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [2B827FB091AC]
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  • Methods in molecular biology
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 1005
http://linked.open...iv/tvurceVysledku
  • Van Eyk, J. E.
  • Šťastná, Miroslava
issn
  • 1940-6029
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/978-1-62703-386-2_18
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