About: 2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing

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About: 2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A combination of two-dimensional high performance liquid chromatography (2D-HPLC) and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS) was used for the analysis of the protein extracts that were reduced, alkylated, and degraded enzymatically without prior protein separation. This so-called “shot-gun approach enabled us to determine glycation sites in one third of the proteins identified in the study and to propose potential glycation markers for fast and efficient monitoring during malting. A combination of two-dimensional high performance liquid chromatography (2D-HPLC) and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS) was used for the analysis of the protein extracts that were reduced, alkylated, and degraded enzymatically without prior protein separation. This so-called “shot-gun approach enabled us to determine glycation sites in one third of the proteins identified in the study and to propose potential glycation markers for fast and efficient monitoring during malting. (en)
Title	2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing 2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing (en)
skos:prefLabel	2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing 2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing (en)
skos:notation	RIV/68081715:_____/10:00348081!RIV11-MSM-68081715
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0570), Z(AV0Z40310501)
http://linked.open...iv/cisloPeriodika	30
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Petry-Podgorska, Inga Žídková, Jitka Flodrová, Dana Bobálová, Janette
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	300888
http://linked.open...ai/riv/idVysledku	RIV/68081715:_____/10:00348081
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	2D-HPLC; MALDI-TOF/TOF mass spectrometry; barley (en)
http://linked.open.../riv/klicoveSlovo	barley 2D-HPLC MALDI-TOF/TOF mass spectrometry
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[2790BA10A78D]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Research Centre for Study of Extract Compounds of Barley and Hop
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	878
http://linked.open...iv/tvurceVysledku	Bobálová, Janette Flodrová, Dana Petry-Podgorska, Inga Žídková, Jitka
http://linked.open...ain/vavai/riv/wos	000284672300014
http://linked.open...n/vavai/riv/zamer	Moderní analytické techniky pro bioanalýzu, ekologii a nanotechnologie
issn	1570-0232
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing 2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing 2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing

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