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Description
| - Ethyl glucuronide (EtG), a metabolite of ethanol, is a marker of recent alcohol consumption. The capillary zone electrophoresis analysis of EtG in model mixtures and human serum is reported using uncoated and coated fused-silica capillaries together with acidic buffers in the pH range between 3.2 and 4.4 and indirect detection. In these approaches, separation of EtG from anionic serum components of high and low concentration is based upon transient isotachophoretic stacking. A buffer composed of 10 mM nicotinic acid and epsilon-aminocaproic acid (pH 4.3) is demonstrated to provide a detection limit for EtG in serum of 0.1 microg/ml, a value that is relevant for clinical and forensic purposes.
- Ethyl glucuronide (EtG), a metabolite of ethanol, is a marker of recent alcohol consumption. The capillary zone electrophoresis analysis of EtG in model mixtures and human serum is reported using uncoated and coated fused-silica capillaries together with acidic buffers in the pH range between 3.2 and 4.4 and indirect detection. In these approaches, separation of EtG from anionic serum components of high and low concentration is based upon transient isotachophoretic stacking. A buffer composed of 10 mM nicotinic acid and epsilon-aminocaproic acid (pH 4.3) is demonstrated to provide a detection limit for EtG in serum of 0.1 microg/ml, a value that is relevant for clinical and forensic purposes. (en)
- Ethyl glucuronid (EtG), metabolit ethanolu, je marker nedávné konzumace alkoholu. Analýza EtG kapilární zónovou elektroforézou v modelových směsích a lidském séru je popsána za použití nepokrytých a pokrytých křemených kapilár za použití kyselých pufrů v rozsahu pH mezi 3.2 a 4.4 a nepřímé detekce. V popsaných postupech je separace EtG od anionických složek séra vysoké a nízké koncentrace založen na efektech přechodné izotachoforézy. Pufr složený z 10 mM nikotinové kyseliny a epsilon-aminokapronové kyseliny (pH 4.3) umožnil dosáhnout detekční limit EtG 0.1 microg/ml, hodnotu, která je použitelná po klinické a soudní účely (cs)
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Title
| - Analysis of ethyl glucuronide in human serum by capillary electrophoresis with sample self-stacking and indirect detection
- Analysis of ethyl glucuronide in human serum by capillary electrophoresis with sample self-stacking and indirect detection (en)
- Analýza etylglukuronidu v lidském séru kapilární elektroforézou se self-stackingem vzorku a nepřímou detekcí (cs)
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skos:prefLabel
| - Analysis of ethyl glucuronide in human serum by capillary electrophoresis with sample self-stacking and indirect detection
- Analysis of ethyl glucuronide in human serum by capillary electrophoresis with sample self-stacking and indirect detection (en)
- Analýza etylglukuronidu v lidském séru kapilární elektroforézou se self-stackingem vzorku a nepřímou detekcí (cs)
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skos:notation
| - RIV/68081715:_____/05:00021363!RIV06-AV0-68081715
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/68081715:_____/05:00021363
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - ethyl glucuronide; capillary electrophoresis; serum (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Chromatography A
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Křivánková, Ludmila
- Gebauer, Petr
- Caslavska, J.
- Thormann, W.
- Malášková, Hana
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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