About: A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Nicotiana tabacum (tobacco, 2n?=?4x?=?48) is an allotetraploid with 24 S-genome chromosomes (from a diploid related to N.?sylvestris) and 24 T-genome chromosomes (from a diploid related to N.?tomentosiformis). The BY-2 suspension cell culture, derived from N.?tabacum cultivar Bright Yellow 2, has been used extensively for research in molecular and cell biology for almost 40 years; a Web of Knowledge search reveals that it has been used over 150 times since 2008 alone, largely for cell cycle and plant physiology studies. However, we show that this culture is unstable and, as with other long-term cultures, exists as a community of cells with different karyotypes reflected in different chromosome numbers, morphologies and distributions of satellite repeats. Nicotiana tabacum (tobacco, 2n?=?4x?=?48) is an allotetraploid with 24 S-genome chromosomes (from a diploid related to N.?sylvestris) and 24 T-genome chromosomes (from a diploid related to N.?tomentosiformis). The BY-2 suspension cell culture, derived from N.?tabacum cultivar Bright Yellow 2, has been used extensively for research in molecular and cell biology for almost 40 years; a Web of Knowledge search reveals that it has been used over 150 times since 2008 alone, largely for cell cycle and plant physiology studies. However, we show that this culture is unstable and, as with other long-term cultures, exists as a community of cells with different karyotypes reflected in different chromosome numbers, morphologies and distributions of satellite repeats. (en)
Title	A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous (en)
skos:prefLabel	A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous (en)
skos:notation	RIV/68081707:_____/12:00389788!RIV13-AV0-68081707
http://linked.open...avai/predkladatel	Biofyzikální ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP501/10/0208)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Matyášek, Roman Kovařík, Aleš Součková, Kamila
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	120512
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/12:00389788
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cell biology; cell cycle; evolution (en)
http://linked.open.../riv/klicoveSlovo	evolution cell biology cell cycle
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[8DA0CCE71089]
http://linked.open...i/riv/nazevZdroje	Botanical Journal of the Linnean Society
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Genome reunions in plants: from DNA to chromosomes and reverse
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	170
http://linked.open...iv/tvurceVysledku	Kovařík, Aleš Leitch, A. R. Matyášek, Roman Lim, K. Y. Součková, Kamila
http://linked.open...ain/vavai/riv/wos	000310079500007
issn	0024-4074
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1095-8339.2012.01280.x
is http://linked.open...avai/riv/vysledek of	A plant culture (BY-2) widely used in molecular and cell studies is genetically unstable and highly heterogeneous

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