About: Voltammetry of osmium-modified DNA at a mercury film electrode application in detecting DNA hybridization

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About: Voltammetry of osmium-modified DNA at a mercury film electrode application in detecting DNA hybridization Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mercury film electrodes (MFE) have recently been used in nucleic acid electrochemical analysis as alternatives to the classical mercury drop ones. DNA modified with osmium tetroxide, 2,2' -bipyridine (Os,bipy) can be detected with a high sensitivity at mercury electrodes via measurements of a catalytic osmiurn signal. In this paper we show that mercury film on a glassy carbon electrode can be used in voltammetric analysis of Os,bipy-modified DNA. Application of the MFE as a detection electrode in double-surface electrochemical DNA hybridization assay involving osmium labeling of target DNA is demonstrated. Mercury film electrodes (MFE) have recently been used in nucleic acid electrochemical analysis as alternatives to the classical mercury drop ones. DNA modified with osmium tetroxide, 2,2' -bipyridine (Os,bipy) can be detected with a high sensitivity at mercury electrodes via measurements of a catalytic osmiurn signal. In this paper we show that mercury film on a glassy carbon electrode can be used in voltammetric analysis of Os,bipy-modified DNA. Application of the MFE as a detection electrode in double-surface electrochemical DNA hybridization assay involving osmium labeling of target DNA is demonstrated. (en) Rtuťové filmové elektrody jsou v současné době využívány v elektrochemické analýze nukleových kyselin jako alternativy ke klasické rtuťové kapkové elektrodě. DNA modifikovaná komplexem oxidu osmičelého s 2,2´-bipyridinem (Os,bipy) může být citlivě stanovena na rtuťových elektrodách s využitím katalytického signálu osmia. V této práci jsme ukázali, že rtuťový film na elektrodě ze skelného uhlíku je vhodný pro voltametrickou analýzu DNA modifikované Os,bipy a může sloužit jako detekční elektroda v dvoupovrchové elektrochemické analýze hybridizace DNA. (cs)
Title	Voltammetry of osmium-modified DNA at a mercury film electrode application in detecting DNA hybridization Voltammetry of osmium-modified DNA at a mercury film electrode application in detecting DNA hybridization (en) Voltametrie DNA modifikované osmiem na rtuťové filmové elektrodě. Využití při detekci hybridizace DNA (cs)
skos:prefLabel	Voltammetry of osmium-modified DNA at a mercury film electrode application in detecting DNA hybridization Voltammetry of osmium-modified DNA at a mercury film electrode application in detecting DNA hybridization (en) Voltametrie DNA modifikované osmiem na rtuťové filmové elektrodě. Využití při detekci hybridizace DNA (cs)
skos:notation	RIV/68081707:_____/04:00104623!RIV/2005/AV0/A17005/N
http://linked.open.../vavai/riv/strany	245;248
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA4004108), P(KJB4004302), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Havran, Luděk Fojta, Miroslav Kostečka, Pavel Pivoňková, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	593287
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/04:00104623
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	osmium; DNA hybridization; mercury film electrode (en)
http://linked.open.../riv/klicoveSlovo	osmium mercury film electrode DNA hybridization
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[A423724843D2]
http://linked.open...i/riv/nazevZdroje	Bioelectrochemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of electrochemical biodetectors for DNA damage Applications of chemical structural probes and electroanalytical methods in DNA damage detection. Development of DNA sensors.
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	63
http://linked.open...iv/tvurceVysledku	Fojta, Miroslav Havran, Luděk Kostečka, Pavel Pivoňková, Hana
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	1567-5394
number of pages	4 (xsd:int)

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