About: Role of tumor suppressor p53 domains in selective binding to supercoiled DNA.

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About: Role of tumor suppressor p53 domains in selective binding to supercoiled DNA. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We showed that bacterially expressed full-length human wild-type p53b(1-393) binds selectively to supercoiled (sc)DNA in sc/linear DNA competition experiments, a process we termed supercoil-selective (SCS) binding. Using p53 deletion mutants and pBluescript scDNA (lacking the p53 recognition sequence) at native superhelix density we demonstrate here that the p53 C- terminal domain (amino acids 347-382) and a p53 oligomeric state are important for SCS binding.Using electron microscopy we observed p53-scDNA nucleoprotein filaments produced by all the C-terminal proteins that displayed SCS binding in the gel electrophoresis experiments. We showed that bacterially expressed full-length human wild-type p53b(1-393) binds selectively to supercoiled (sc)DNA in sc/linear DNA competition experiments, a process we termed supercoil-selective (SCS) binding. Using p53 deletion mutants and pBluescript scDNA (lacking the p53 recognition sequence) at native superhelix density we demonstrate here that the p53 C- terminal domain (amino acids 347-382) and a p53 oligomeric state are important for SCS binding.Using electron microscopy we observed p53-scDNA nucleoprotein filaments produced by all the C-terminal proteins that displayed SCS binding in the gel electrophoresis experiments. (en)
Title	Role of tumor suppressor p53 domains in selective binding to supercoiled DNA. Role of tumor suppressor p53 domains in selective binding to supercoiled DNA. (en)
skos:prefLabel	Role of tumor suppressor p53 domains in selective binding to supercoiled DNA. Role of tumor suppressor p53 domains in selective binding to supercoiled DNA. (en)
skos:notation	RIV/68081707:_____/02:17023181!RIV/2003/GA0/A17003/N
http://linked.open.../vavai/riv/strany	4966;4974
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/02/0831), P(GA301/99/D078), P(IAA4004110), P(IBS5004009), P(NC5343), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	22
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Paleček, Emil Pečinka, Petr Fojta, Miroslav Paleček, Jan Brázdová, Marie Billová, Sabina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	662669
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/02:17023181
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	tumor suprressor protein p53; supercoiled DNA; DNA-protein binding (en)
http://linked.open.../riv/klicoveSlovo	supercoiled DNA DNA-protein binding tumor suprressor protein p53
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[646EE83206CF]
http://linked.open...i/riv/nazevZdroje	Nucleic Acids Research
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Interactions of p53 protein and its homologues with DNA and their role in malignant transformation Role of the p53 domains and the oligomerization state of the protein in its molecular interactions Untraditional therapeutic approaches in oncology Interaction of tumor supressor protein p53 with damaged DNA and with DNA lesions induced by anti-cancer drugs Binding of tumor suppressor protein p53 to DNA. Effect of DNA supercoling and protein postsynthetic modification
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	30
http://linked.open...iv/tvurceVysledku	Brázdová, Marie Paleček, Emil Paleček, Jan Jovin, T. M. Vojtěšek, B. Pečinka, Petr Billová, Sabina Cherny, D. I. Fojta, Miloslav
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	0305-1048
number of pages	9 (xsd:int)

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