About: Intestinal bacterial population of healthy rats during the administration of chitosan and chitooligosaccharides

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The effect of the administration of chitosan (CS) and chitooligosaccharides (COS) on rat fecal microbiota was analyzed in this study. The profile of total bacterial population was monitored during 3 weeks of CS or COS application using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons. Quantitative PCR was used for monitoring possible changes in the levels of total bacteria and the levels of individual bacterial groups: Bifidobacteria, Clostridium leptum, Enterobacteriaceae, Lactobacillus-Streptococcus-Enterobacter, and Bacteroides-Prevotella. The DGGE profiles revealed a high complexity and individuality of each tested subject, and variations in the composition of band pattern were observed. CS or COS per os administration changed the profile and structure of the microbial ecosystem of the gastrointestinal tract of healthy rats. COS have, in most cases, an opposite effect compared with CS; only the Bacteroides-Prevotella bacterial group and Enterobacteriaceae were influenced in the same way. The Bifidobacteria group was not influenced by the administration CS and COS. The effect of the administration of chitosan (CS) and chitooligosaccharides (COS) on rat fecal microbiota was analyzed in this study. The profile of total bacterial population was monitored during 3 weeks of CS or COS application using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons. Quantitative PCR was used for monitoring possible changes in the levels of total bacteria and the levels of individual bacterial groups: Bifidobacteria, Clostridium leptum, Enterobacteriaceae, Lactobacillus-Streptococcus-Enterobacter, and Bacteroides-Prevotella. The DGGE profiles revealed a high complexity and individuality of each tested subject, and variations in the composition of band pattern were observed. CS or COS per os administration changed the profile and structure of the microbial ecosystem of the gastrointestinal tract of healthy rats. COS have, in most cases, an opposite effect compared with CS; only the Bacteroides-Prevotella bacterial group and Enterobacteriaceae were influenced in the same way. The Bifidobacteria group was not influenced by the administration CS and COS. (en)
Title	Intestinal bacterial population of healthy rats during the administration of chitosan and chitooligosaccharides Intestinal bacterial population of healthy rats during the administration of chitosan and chitooligosaccharides (en)
skos:prefLabel	Intestinal bacterial population of healthy rats during the administration of chitosan and chitooligosaccharides Intestinal bacterial population of healthy rats during the administration of chitosan and chitooligosaccharides (en)
skos:notation	RIV/67985904:_____/12:00387240!RIV13-GA0-67985904
http://linked.open...avai/predkladatel	Ústav živočišné fyziologie a genetiky AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GA310/09/1407)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Koppová, Ingrid Šimůnek, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	142723
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/12:00387240
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	16S ribosomal RNA; real-time PCR; fecal samples (en)
http://linked.open.../riv/klicoveSlovo	16S ribosomal RNA fecal samples real-time PCR
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[2FE12A2A6415]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Chitinolytic enzymes from Clostridium paraputrificum inhabiting the human gastrointestinal tract; structure and biomedical implications.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Koppová, Ingrid Šimůnek, Jiří Bureš, M.
http://linked.open...ain/vavai/riv/wos	000305980400011
issn	0015-5632
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s12223-012-0129-2
is http://linked.open...avai/riv/vysledek of	Intestinal bacterial population of healthy rats during the administration of chitosan and chitooligosaccharides

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